H1404

Hepatocyte Growth Factor human

>95% (SDS-PAGE), recombinant, expressed in Baculovirus infected High-5 cells, lyophilized powder, suitable for cell culture

• 동의어(들): HGF, Hepatopoietin A, Scatter Factor
• EC Number: 214-275-1
• MDL number: MFCD00217422
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• product name: Hepatocyte Growth Factor human, HGF, recombinant, expressed in Baculovirus infected High-5 cells, suitable for cell culture
• 생물학적 소스: human
• Quality Level: 200
• 재조합: expressed in Baculovirus infected High-5 cells
• 분석: >95% (SDS-PAGE)
• 형태: lyophilized powder
• 효능: 1.0-20.0 ng/mL
• 품질: endotoxin tested
• 분자량: ~80 kDa
• 포장: pkg of 5x5 μg; pkg of 5 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 기술: cell culture | mammalian: suitable
• 불순물: <1.0 EU/μg
• UniProt 수납 번호: P14210
• 저장 온도: −20°C
• 유전자 정보: human ... HGF(3082)

설명

생화학적/생리학적 작용

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.

기타 정보

The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.

물리적 형태

Lyophilized from a 0.2 μm filtered solution in 20 mM NaH₂PO₄ and 500 mM NaCl, pH 7.0 with 50 μg BSA per 1 μg HGF

제조 메모

Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. After reconstitution, this solution can be stored at 2-8 °C for up to one month or at -20 °C to -70 °C for up to three months. Us a manual defrost freezer and avoid repeated freeze-thaw cycles.

분석 메모

The biological activity is measured by its ability to induce IL-11 secretion by Saos-2 human osieosarcoma cells.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Gloves