L5158

Leukemia Inhibitory Factor from mouse

≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture

• 동의어(들): Mouse LIF, Mouse Leukemia Inhibitory Factor, Murine LIF, mLIF, LIF
• MDL number: MFCD00283681
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• product name: Leukemia Inhibitory Factor from mouse, LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution, suitable for cell culture
• 재조합: expressed in E. coli
• Quality Level: 200
• 분석: ≥95% (SDS-PAGE)
• 형태: buffered aqueous solution
• 품질: endotoxin tested
• 기술: cell culture | mammalian: suitable
• 저장 온도: 2-8°C

설명

애플리케이션

Leukemia Inhibitory Factor from mouse has been used:

• as a component of enriched co-culture media
• as a supplement in Dulbecco′s modified eagle′s medium (DMEM)/F12 medium for culturing heart cells
• as a component in maintenance medium for maintaining embryonic stem cells
• to treat ovaries in ovarian culture

생화학적/생리학적 작용

Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein originally described to inhibit the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Other activities were later identified to LIF, which is known by a variety of synonyms, including DIF, D-factor, DIA, DRF, CNDF, HILDA, HSF-III, and MLPLI. Human LIF exerts its actions through a receptor comprising a 190 kDa LIF-binding α-chain (130 kDa, mouse) and a 130 kDa signal-transducing β-chain (gp130), which is shared with CNTF, OSM, L-6 and IL-11. As such, it is a member of the gp130 family of the cytokine receptor superfamily. LIF receptors have been identified on several cells, including monocytes, liver, placenta and embryonic stem cells. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, depending on the source, but absence of glycosylation appears not to affect its biological activity. A single gene encodes LIF, which is secreted as a single chain glycoprotein containing 180 amino acids for human or mouse with a conserved disulfide bond. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.

물리적 형태

Solution in phosphate buffered saline containing 0.02% TWEEN^® 20

분석 메모

The proliferative activity of mouse LIF is measured in culture using the M1 murine cell line.

법적 정보

TWEEN is a registered trademark of Croda International PLC

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)