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LIF1010

Sigma-Aldrich

Leukemia Inhibitory Factor human

10 µg, human recombinant LIF protein, expressed in E. coli, suitable for stem cell culture

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Synonym(s):
Human LIF, Human Leukemia Inhibitory Factor, LIF, hLIF
eCl@ss:
32160405
NACRES:
NA.75

biological source

human

Quality Level

Assay

>95% (HPLC and SDS-PAGE)

form

liquid

specific activity

≥1 x 10(E8) U/mg

manufacturer/tradename

Chemicon®

concentration

10 μg/mL

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 ng/mg Endotoxin (of LIF)

input

sample type neural stem cell(s)
sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... LIF(3976)

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This Item
LIF1005LIF1050L5283
assay

>95% (HPLC and SDS-PAGE)

assay

>95% (HPLC and SDS-PAGE)

assay

>95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

form

liquid

form

liquid

form

liquid

form

buffered aqueous solution (pH 7.4)

specific activity

≥1 x 10(E8) U/mg

specific activity

≥1 x 10(E8) U/mg

specific activity

≥1 x 10(E8) U/mg

specific activity

-

concentration

10 μg/mL

concentration

10 μg/mL

concentration

100 μg/mL

concentration

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

General description

Human LIF is a 19.7 kDa protein containing 181 amino acid residues. The non-glycosylated, E. coli expressed, recombinant human LIF is indistinguishable from native LIF in its biological activities in vitro. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid control. Human LIF is equally active on both human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells, than hLIF.
Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo.
Product Source: LIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moeity with thrombin and purified by HPLC chromatography.

Application

10 µg, human recombinant LIF protein, expressed in E. coli, suitable for stem cell culture

Unit Definition

Specific Activity: where 50 units is defined as the amount of human LIF required to induce differentiation in 50% of the M1 colonies in 1 mL agar cultures.

Physical form

Liquid in PBS, pH 7.4 and 0.02% Tween 20. No preservativies added.

Storage and Stability

Maintain at 2-8°C for 6 - 12 months. Further dilutions should be made into buffer or medium to which protein (e.g., 1% BSA) or Tween 20 has been added.



Manufactured by CHEMICON International, Inc. LIF is protected under US Patent nos. 5,443,825, 5,750,654 and 6,261,548, European Patent no. 0285 448 and related foreign patents and is not available for resale.

Analysis Note

Specific Activity: The activity of human LIF is determined by the ability to induce differentiation of M1 myeloid leukemic cells.The minimum detectable concentration of human LIF in this assay is 0.5 ng/mL
Tested negative in both aseptic and microplasmic tests.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

LABEL LICENSE - USE RESTRICTED TO RESEARCH USE ONLY

Leukemia Inhibitory Factor ("LIF") is protected by US Patents No. 5,443,825 and 5,750,654 and Canada Patent No. 1,341,581 and 1,341,469 owned by AMRAD Corporation Ltd. and licensed exclusively to Millipore for research uses. The use of this product is limited for research uses only and not for any commercial purposes, such as making or developing other research products from this product or use of this product as a component in a research kit. This product is not to be used in humans or for any diagnostic purposes.

The purchaser may refuse to accept the conditions of this label license by returning this product unopened and will receive a full refund of the purchase price paid.

For-profit entities or commercial research entities purchasing this product shall be required to execute a separate commercial license with Millipore within three months of purchase. Researchers may not transfer this product or its derivatives to researchers performing commercial research at other Nonprofit Organizations, or to For-Profit Organizations, without the express written consent of Millipore, and without those entities having an express license from Millipore for the use of the product. Other than as specifically provided herein, a transferee of this product shall have no right to transfer this product, or its derivatives to any other person or entity.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

Leukemia Inhibitory Factor (LIF) Protein

Leukemia Inhibitory Factor (LIF) is a stem cell growth factor used for the in vitro culture of pluripotent mouse embryonic stem cells (ES cells).

Pluripotent and Multipotent Stem Cells

Read types of stem cells including multipotent stem cells, Pluripotent stem cells and iPSCs and their applications in basic stem cell research, stem cell therapy and disease modelling

Protocols

Neural Stem Cell Culture Protocols

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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