L5158
Leukemia Inhibitory Factor from mouse
LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution, suitable for cell culture
Synonym(s):
Mouse LIF, Mouse Leukemia Inhibitory Factor, Murine LIF, mLIF, LIF
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
Recommended Products
recombinant
expressed in E. coli
Quality Level
assay
≥95% (SDS-PAGE)
form
buffered aqueous solution
quality
endotoxin tested
technique(s)
cell culture | mammalian: suitable
storage temp.
2-8°C
Looking for similar products? Visit Product Comparison Guide
Application
Leukemia Inhibitory Factor from mouse has been used:
- as a component of enriched co-culture media
- as a supplement in Dulbecco′s modified eagle′s medium (DMEM)/F12 medium for culturing heart cells
- as a component in maintenance medium for maintaining embryonic stem cells
- to treat ovaries in ovarian culture
Biochem/physiol Actions
Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein originally described to inhibit the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Other activities were later identified to LIF, which is known by a variety of synonyms, including DIF, D-factor, DIA, DRF, CNDF, HILDA, HSF-III, and MLPLI. Human LIF exerts its actions through a receptor comprising a 190 kDa LIF-binding α-chain (130 kDa, mouse) and a 130 kDa signal-transducing β-chain (gp130), which is shared with CNTF, OSM, L-6 and IL-11. As such, it is a member of the gp130 family of the cytokine receptor superfamily. LIF receptors have been identified on several cells, including monocytes, liver, placenta and embryonic stem cells. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, depending on the source, but absence of glycosylation appears not to affect its biological activity. A single gene encodes LIF, which is secreted as a single chain glycoprotein containing 180 amino acids for human or mouse with a conserved disulfide bond. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.
Physical form
Solution in phosphate buffered saline containing 0.02% TWEEN® 20
Analysis Note
The proliferative activity of mouse LIF is measured in culture using the M1 murine cell line.
Legal Information
TWEEN is a registered trademark of Croda International PLC
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
C-Kit Positive Cardiac Stem Cells and Bone Marrow-Derived Mesenchymal Stem Cells Synergistically Enhance Angiogenesis and Improve Cardiac Function After Myocardial Infarction in a Paracrine Manner
Bao L, et al.
Journal of Cardiac Failure, 23(5), 403-415 (2017)
Suppressor of cytokine signaling 4 (SOCS4): moderator of ovarian primordial follicle activation
Sutherland JM, et al.
Journal of Cellular Physiology, 227(3), 1188-1198 (2012)
LIF-immobilized nonwoven polyester fabrics for cultivation of murine embryonic stem cells
Cetinkaya G, et al.
Journal of Biomedical Materials Research Part A, 81(4), 911-919 (2007)
Renée F de Pooter et al.
Methods in molecular biology (Clifton, N.J.), 330, 113-121 (2006-07-19)
Mature hematopoietic cells, like all other terminally differentiated lineages, arise during ontogeny via a series of increasingly restricted intermediates. Hematopoietic progenitors have their origin in the mesoderm, which gives rise to hemangioblasts that can differentiate into endothelial or endocardial precursors
Cristian Soitu et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 7(23), 2001854-2001854 (2020-12-12)
There is an unmet demand for microfluidics in biomedicine. This paper describes contactless fabrication of microfluidic circuits on standard Petri dishes using just a dispensing needle, syringe pump, three-way traverse, cell-culture media, and an immiscible fluorocarbon (FC40). A submerged microjet
Articles
Leukemia Inhibitory Factor (LIF) is a stem cell growth factor used for the in vitro culture of pluripotent mouse embryonic stem cells (ES cells).
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service