M0440

Mitomycin C from Streptomyces caespitosus

meets USP testing specifications

• 동의어(들): Mitomycin
• 실험식(Hill 표기법): C₁₅H₁₈N₄O₅
• CAS Number: 50-07-7
• Molecular Weight: 334.33
• Beilstein: 7231816
• EC Number: 200-008-6
• MDL number: MFCD00078109
• UNSPSC 코드: 12352203
• PubChem Substance ID: 24896554
• NACRES: NA.76

속성

• 생물학적 소스: Streptomyces caespitosus
• Quality Level: 200
• 색상: blue-violet
• solubility: H₂O: 40 mg/mL, clear to very slightly hazy, colorless; H₂O: soluble
• 항생제 활성 스펙트럼: Gram-negative bacteria; Gram-positive bacteria
• 동작 모드: DNA synthesis | interferes
• 저장 온도: 2-8°C
• SMILES string: [H][C@]12CN3C4=C([C@@H](COC(N)=O)[C@@]3(OC)[C@@]1([H])N2)C(=O)C(N)=C(C)C4=O
• InChI: 1S/C15H18N4O5/c1-5-9(16)12(21)8-6(4-24-14(17)22)15(23-2)13-7(18-13)3-19(15)10(8)11(5)20/h6-7,13,18H,3-4,16H2,1-2H3,(H2,17,22)/t6-,7+,13+,15-/m1/s1
• InChI key: NWIBSHFKIJFRCO-WUDYKRTCSA-N

설명

일반 설명

Chemical structure: aziridine

애플리케이션

Mitomycin C is produced by a strain of actinomyces, Streptomyces caespitosus. It contains three anticancer moieties, quinine, urethane, and aziridine groups. It is used to generate mitotically inactive feeder cells in cell culture systems, such as the mitotically inactive fibroblasts used in embryonic stem cell systems.

생화학적/생리학적 작용

Mode of Action: This product is an alkylating agent that specifically targets the guanine nucleoside sequence 5′-CpG-3′. It inhibits DNA synthesis by covalently reacting with DNA, forming crosslinks between complementary strands of DNA. This interaction prevents separation of complementary DNA strands, inhibiting DNA replication.

Antimicrobial Spectrum: Mitomycin C has strong antitumor activity, especially against Ehrlich ascites tumor cells, and strong bactericidal action against gram-positive and gram-negative bacteria.

주의사항

Stock solutions should be filter sterilized and stored at 2-8 °C in the dark. Solutions at pH 6-9 can be stored at 0-5 °C for up to a week, but if a precipitate forms, a fresh solution should be prepared - the precipitated solution has been proven toxic to cells.

제조 메모

Mitomycin C is soluble in water at .5 mg/mL, with a pH of 6.0-7.5. It undergoes rapid degradation in acidic solutions with pH<6, and is mostly likely to retain activity in solutions with a pH between 6-9.

안전성 정보

• 픽토그램: GHS06,GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H300,H351
• 예방조치 성명서: P201 - P301 + P310 + P330
• Hazard Classifications: Acute Tox. 2 Oral - Carc. 2
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges