추천 제품
사용
sufficient for 100 colorimetric or fluorometric tests
응용 분야
cosmetics
food and beverages
검출 방법
colorimetric
fluorometric
관련 질환(들)
endocrinological disorders, diabetes
저장 온도
−20°C
일반 설명
Sucrose is a disaccharide that is hydrolyzed to glucose and fructose by invertase. Glucose is the primary carbohydrate utilized for energy during cellular respiration. Upon cellular uptake, glucose can be shunted towards breakdown via glycolysis or polymerized for energy storage.
애플리케이션
Glucose and sucrose assay kit has been used to:
- determine sucrose, aerobic glycolysis and pyruvate kinase activity
- determine protein and sugar in strawberries
- determine plasma insulin and glucose levels in mice
- analyze plasma glucose and plasma lactate and to quantify glucose/sucrose.
적합성
Suitable for use with various biological samples including serum, plasma, other body fluids, food, and growth media.
원리
In this assay kit, glucose is oxidized via glucose oxidase resulting in a colorimetric (570 nm)/ fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the glucose present. To measure sucrose, invertase is added to the reaction to convert the sucrose to glucose and fructose. The free glucose can be subtracted from the total glucose to give the concentration of sucrose present. This kit has a linear range of detection between 0.2-1.0 nmole of glucose for the fluorometric assay and 2-10 nmoles of glucose for the colorimetric assay.
신호어
Danger
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Aquatic Chronic 3 - Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
Flash Point (°F)
188.6 °F - closed cup
Flash Point (°C)
87 °C - closed cup
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
Fatemeh Kazeminasab et al.
Nutrition & metabolism, 18(1), 75-75 (2021-07-22)
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Bertrand T F, et al.
International Journal of Current Microbiology and Applied Sciences, 6(6), 3286-3298 (2017)
Rates of Spontaneous Cleavage of Glucose, Fructose, Sucrose, and Trehalose in Water, and the Catalytic Proficiencies of Invertase and Trehalas
Richard W and Yang Y
Journal of the American Chemical Society, 130(24), 7548-7549 (2008)
Joshua T Huffines et al.
Scientific reports, 10(1), 19661-19661 (2020-11-14)
Polymicrobial interactions in dental plaque play a significant role in dysbiosis and homeostasis in the oral cavity. In early childhood caries, Streptococcus mutans and Candida albicans are often co-isolated from carious lesions and associated with increased disease severity. Studies have
Swee-Suak Ko et al.
International journal of molecular sciences, 21(17) (2020-08-30)
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