MAK013

Glucose and Sucrose Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• 동의어(들): Glucose and Sucrose Colorimetric/Fluorometric Assay Kit
• UNSPSC 코드: 12161503
• NACRES: NA.84

속성

• 사용: sufficient for 100 colorimetric or fluorometric tests
• 응용 분야: cosmetics; food and beverages
• 검출 방법: colorimetric; fluorometric
• 관련 질환(들): endocrinological disorders, diabetes
• 저장 온도: −20°C

설명

일반 설명

Sucrose is a disaccharide that is hydrolyzed to glucose and fructose by invertase.[1] Glucose is the primary carbohydrate utilized for energy during cellular respiration.[2] Upon cellular uptake, glucose can be shunted towards breakdown via glycolysis or polymerized for energy storage.[2]

애플리케이션

Glucose and sucrose assay kit has been used to:

• determine sucrose,[3] aerobic glycolysis and pyruvate kinase activity[4]
• determine protein and sugar in strawberries[5]
• determine plasma insulin and glucose levels in mice[6]
• analyze plasma glucose and plasma lactate[7] and to quantify glucose/sucrose.[8]

적합성

Suitable for use with various biological samples including serum, plasma, other body fluids, food, and growth media.

원리

In this assay kit, glucose is oxidized via glucose oxidase resulting in a colorimetric (570 nm)/ fluorometric (λ_ex = 535 nm/λ_em = 587 nm) product, proportional to the glucose present. To measure sucrose, invertase is added to the reaction to convert the sucrose to glucose and fructose. The free glucose can be subtracted from the total glucose to give the concentration of sucrose present. This kit has a linear range of detection between 0.2-1.0 nmole of glucose for the fluorometric assay and 2-10 nmoles of glucose for the colorimetric assay.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334,H412
• 예방조치 성명서: P261 - P273 - P284 - P501
• Hazard Classifications: Aquatic Chronic 3 - Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): 188.6 °F - closed cup
• Flash Point (°C): 87 °C - closed cup