MilliporeSigma
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GAHK20

Supelco

Glucose (HK) Assay Kit

sufficient for 20 assays

NACRES:
NA.84

usage

sufficient for 20 assays

Quality Level

analyte chemical class(es)

sugars (glucose)

technique(s)

photometry: suitable

application(s)

food and beverages
general analytical

storage temp.

2-8°C

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technique(s)

photometry: suitable

technique(s)

photometry: suitable

technique(s)

photometry: suitable

technique(s)

photometry: suitable

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

analyte chemical class(es)

sugars (glucose)

analyte chemical class(es)

sugars (fructose)

analyte chemical class(es)

sugars

analyte chemical class(es)

-

application(s)

food and beverages
general analytical

application(s)

food and beverages
general analytical

application(s)

food and beverages
general analytical

application(s)

food and beverages
general analytical

Application

This kit is for the quantitative, enzymatic determination of glucose in food and other material. Glucose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phospho-gluconate in the presence of oxidized nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to glucose concentration.

Kit Components Only

Product No.
Description

  • glucose (HK) assay reagent 20 mL

  • glucose standard .5 mL

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


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25G
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1000309185

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Rebecca A Haeusler et al.
Nature communications, 5, 5190-5190 (2014-10-14)
Insulin integrates hepatic glucose and lipid metabolism, directing nutrients to storage as glycogen and triglyceride. In type 2 diabetes, levels of the former are low and the latter are exaggerated, posing a pathophysiologic and therapeutic conundrum. A branching model of
Louis-Philippe Bernier et al.
Nature communications, 11(1), 1559-1559 (2020-03-28)
Microglia are highly motile cells that continuously monitor the brain environment and respond to damage-associated cues. While glucose is the main energy substrate used by neurons in the brain, the nutrients metabolized by microglia to support surveillance of the parenchyma
Bo-Ruei Chen et al.
Experimental gerontology, 44(8), 493-502 (2009-05-05)
We describe a new chronological lifespan (CLS) assay for the yeast Schizosaccharomyces pombe. Yeast CLS assays monitor the loss of cell viability in a culture over time, and this new assay shows a continuous decline in viability without detectable regrowth
Kuei-Ching Hsiung et al.
Biomedical journal, 43(1), 32-43 (2020-03-24)
CDGSH iron sulfur domain-containing protein 1 (CISD-1) belongs to the CISD protein family that is evolutionary conserved across different species. In mammals, CISD-1 protein has been implicated in diseases such as cancers and diabetes. As a tractable model organism to
Kunst, A., et al.
Methods of Enzymatic Analysis, 163-172 (1984)

Articles

Nuclear Magnetic Resonance (NMR) Spectroscopy Analysis of Glycans

Nuclear magnetic resonance (NMR) spectroscopy measures the extent to which a glycan or other molecule distorts a magnetic field.

Protocols

Enzymatic Food Analysis

Enzymatic methods for food analysis are highly specific and offer considerable time and cost savings over other methods, especially from the sample preparation standpoint. We offer a wide variety of convenient kits and reagents for rapid and reliable enzymatic food analysis.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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