추천 제품
사용
sufficient for 25 preparations
Quality Level
환경친화적 대안 제품 특성
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기술
DNA extraction: suitable
환경친화적 대안 카테고리
저장 온도
15-25°C
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
The GenElute HP Endotoxin-Free Plasmid Maxiprep Kit offers a simple and rapid method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. The kit uses a vacuum format with a filter column for the rapid clearing of the bacterial lysate and a silica column for capturing plasmid DNA. A proprietary solution binds plasmid DNA to the binding column while preventing endotoxins from adsorbing. The technology allows for the user to consistently achieve levels of endotoxin that are less than 0.1 endotoxin units per μg of plasmid DNA.
High-quality, endotoxin-free DNA is ready for immediate use for the most demanding applications including transfection with endotoxin-sensitive cells.
High-quality, endotoxin-free DNA is ready for immediate use for the most demanding applications including transfection with endotoxin-sensitive cells.
애플리케이션
GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit has been used in the isolation of plasmid from swine liver and from competent Escherichia coli cells.
특징 및 장점
- Up to 1.2 mg of high-copy plasmid DNA with endotoxin levels of ≤0.1 EU/μg
- Only 35 minutes from pelleted cells to purified plasmid
- Convenient vacuum format
원리
An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis. The lysate is clarified by filtration followed by the addition of a binding solution that has been optimized for endotoxin-free plasmid preparations. The plasmid DNA is then captured on silica, while endotoxins are prevented from adsorbing to the membrane. Two wash steps remove contaminants. Finally, the bound DNA is eluted in endotoxin-free water. The recovered plasmid DNA is predominately in its supercoiled form. Genomic DNA and RNA are below detectable levels by ethidium bromide stained agarose gel electrophoresis.
기타 정보
For additional information, please see www.sigma-aldrich.com/genelutehp.
법적 정보
GenElute is a trademark of Sigma-Aldrich Co. LLC
관련 제품
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
표적 기관
Central nervous system
Storage Class Code
3 - Flammable liquids
Flash Point (°F)
77.0 °F - closed cup
Flash Point (°C)
25 °C - closed cup
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
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관련 콘텐츠
GenElute HP Plasmid Maxiprep Kit isolates endotoxin-free plasmid DNA in 35 minutes, meeting industry standards.
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