P1860

Protease Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases, for use in tissue culture media, DMSO solution

• 동의어(들): Protease Inhibitor Solution, protease inhibitor
• EC Number: 200-664-3
• MDL number: MFCD00677817
• UNSPSC 코드: 12352200
• NACRES: NA.77
• 양식: DMSO solution
• 저장 온도: −20°C

속성

• 제품명: Protease Inhibitor Cocktail, for use in tissue culture media, DMSO solution
• Quality Level: 200
• 양식: DMSO solution
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

Protease Inhibitor Cocktail demonstrated non-toxicity towards the following cell lines after 48 hours exposure:
A431, CHO, COS, HepG2, and HeLa adherent cell lines
Jurkat and HL-60 cell lines grown in suspension

The Protease Inhibitor Cocktail is a mixture of protease inhibitors designed to prevent proteolytic degradation of secreted proteins in tissue culture media.

특이성

Inhibits serine, cysteine, aspartic proteases, and aminopeptidases

애플리케이션

Protease Inhibitor Cocktail has been used:

• as a supplement in supernatants and lysates for ex vivo infection experiments using human precision-cut lung slices (PCLS)
• to inhibit proteolysis by trypsin to prove that proteolysis is responsible for restoration of catalysis of corona-inhibited nanozymes
• in trypsin solution for trypsin-inhibited studies
• as a component in radioimmunoprecipitation assay (RIPA) buffer to lyse leukocytes for western blot

This product is specifically optimized for use in tissue culture media and can be added after 48 hours of exposure to fresh medium to ensure the continued inhibition of proteases.

생화학적/생리학적 작용

Protease inhibitor cocktail is designed for use in tissue culture media. It is recommended as an additive to tissue culture media to safeguard against the degradation of secreted proteins originating from the cultured tissue.

특징 및 장점

• Broad specificity
• Non-toxic: After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.
• Contains no metal chelators: Ensures compatibility with downstream applications
• Convenient packaging: 1 mL in glass bottle
• Easy to use: Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

성분

Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A

주의사항

After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.

수량

Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

물리적 형태

Solution in DMSO (D 2650, Hybri-Max).

기타 정보

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): 188.6 °F
• Flash Point (°C): 87 °C