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P8849

Sigma-Aldrich

Protease Inhibitor Cocktail

for use in purification of Histidine-tagged proteins, DMSO solution

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MDL number:
NACRES:
NA.77

biological source

synthetic

Quality Level

form

DMSO solution

storage temp.

−20°C

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This Item
P8340P9599P1860
vibrant-m

P8849

Protease Inhibitor Cocktail

vibrant-m

P8340

Protease Inhibitor Cocktail

vibrant-m

P9599

Protease Inhibitor Cocktail

vibrant-m

P1860

Protease Inhibitor Cocktail

form

DMSO solution

form

DMSO solution

form

DMSO solution

form

DMSO solution

biological source

synthetic

biological source

bovine lung

biological source

-

biological source

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

DMSO solution designed for use in the purification of histidine-tagged proteins.

The cocktail contains a mixture of inhibitors that specifically target serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.

Specificity

Inhibits serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.

Application

The cocktail has been optimized and tested for isolation of histidine-tagged proteins, with chelating agents omitted for compatibility with IMAC applications.

It is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells.

Features and Benefits

Specifically formulated for histidine-tagged protein purification.

Targets multiple types of proteases, ensuring comprehensive inhibition of protease activity.

Compatibility with IMAC applications due to omission of chelating agents.

Supplied in convenient packaging options.

Components

AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon

Quantity

One mL is recommended for the inhibition of proteases extracted from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells in a total volume of 100 ml.

This protease inhibitor cocktail has been optimized and tested for histidine-tagged proteins

Preparation Note

This product is supplied as a clear solution in DMSO. One mL of solution is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of E. coli cells or 10 g of baculovirus-infected cells.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

185.0 °F - closed cup

flash_point_c

85 °C - closed cup


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