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Protease Inhibitor Cocktail III

EDTA-Free, liquid, for the inhibition of aspartic, cysteine, and serine proteases and aminopeptidases in mammalian cells and tissues.

Synonym(s):

EDTA free Protease inhibitor, EDTA-Free Protease Inhibitor Mix, Protease inhibitor cocktail III

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.77

product name

Protease Inhibitor Cocktail Set III, EDTA-Free, Protease inhibitor cocktail III, EDTA-free for inhibiting aspartic, cysteine, and serine proteases as well as aminopeptidases in mammalian cells and tissues.

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

shipped in

wet ice

storage temp.

−20°C

General description

Note: available as 1 x 1 ml or as a set of 5 x 1 ml.
This protease inhibitor cocktail set is a specially formulated cocktail of six protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases. This cocktail is recommended for use with mammalian cells and tissue extracts, but is also suitable for bacterial extracts. It is provided as a single vial or as a set of 5 vials. Each vial contains 1 ml protease inhibitor cocktail solubilized in DMSO with a small amount of water.

Specificity

Inhibits a broad spectrum of serine proteases, cysteine proteases, and aspartic proteases, as well as aminopeptidases.

Application

Protease Inhibitor Cocktail Set III, EDTA-Free has been used:
  • as a component in CelLytic MT cell lysis reagent or lysis buffer to homogenize kidney organoids for immunoblot analysis
  • as a component in homogenization buffer to homogenize minced mouse quadriceps femoris muscle tissue
  • in lysis buffer to extract proteins for immunoblotting

Biochem/physiol Actions

Protease inhibitor cocktails and specialized protease inhibitors play a crucial role in various proteomic studies conducted by scientists. These studies encompass protein expression and characterization, biomarker discovery, identification of post-translational modifications, protein expression profiling, and quantitative protein measurement. The inclusion of a protease inhibitor cocktail or specific protease inhibitor guarantees the preservation of protein integrity, allowing for accurate downstream analysis and comprehensive sample characterization.

Features and Benefits

  • EDTA-Free: recommended for IMAC applications.
  • Flexible: compatible with bacterial extracts, mammalian cell extracts, and tissue extracts.
  • Fast: no waiting time for tablets to dissolve.
  • Economical: ready-to-use format reduces wastage.
  • Effective: one ml is sufficient for approximately 20g of tissue.
  • Complete: contains six protease inhibitors to inhibit aspartic, cysteine, serine proteases, and aminopeptidases.

Warning

Toxicity: Irritant (B)

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

188.6 °F - (refers to pure substance)

flash_point_c

87 °C - (refers to pure substance)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Resin-assisted enrichment of thiols as a general strategy for proteomic profiling of cysteine-based reversible modifications
Guo J, et al.
Nature Protocols, 9, 64-75 (2014)
Zhengxia Wang et al.
Allergy, asthma & immunology research, 12(5), 859-876 (2020-07-09)
CD4⁺T cells are essential in the pathogenesis of allergic asthma. We have previously demonstrated that microRNA-1165-3p (miR-1165-3p) was significantly reduced in T-helper type (Th) 2 cells and that miR-1165-3p was a surrogate marker for atopic asthma. Little is known about
Wei-Ling Wang et al.
American journal of physiology. Renal physiology, 318(4), F956-F970 (2020-02-25)
Aquaporin-2 (AQP2) is a vasopressin-regulated water channel protein responsible for osmotic water reabsorption by kidney collecting ducts. In response to vasopressin, AQP2 traffics from intracellular vesicles to the apical plasma membrane of collecting duct principal cells, where it increases water
Functional characterization of ion channels expressed in kidney organoids derived from human induced pluripotent stem cells
Montalbetti N, et al.
American Journal of Physiology: Renal Physiology, 323(4), F479-F491 (2022)
Larissa Gomes Dos Reis et al.
Drug development and industrial pharmacy, 46(3), 427-442 (2020-02-20)
The combination of nanoparticles (NPs) and cell-penetrating peptide (CPP) represents a new opportunity to develop plasmid DNA (pDNA) delivery systems with desirable properties for lung delivery. In this study, poly(lactide-co-glycolide) (PLGA) NPs containing pDNA were formulated with and without CPP

Articles

Learn tips and tricks for how to properly use inhibitors including how to select the right inhibitor and how to plan experiments with inhibitors.

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