P1860
Protease Inhibitor Cocktail
DMSO solution, for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases, for use in tissue culture media, DMSO solution
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product name
Protease Inhibitor Cocktail, for use in tissue culture media, DMSO solution
Quality Level
form
DMSO solution
shipped in
dry ice
storage temp.
−20°C
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Related Categories
General description
A431, CHO, COS, HepG2, and HeLa adherent cell lines
Jurkat and HL-60 cell lines grown in suspension
Specificity
Application
- as a supplement in supernatants and lysates for ex vivo infection experiments using human precision-cut lung slices (PCLS)
- to inhibit proteolysis by trypsin to prove that proteolysis is responsible for restoration of catalysis of corona-inhibited nanozymes
- in trypsin solution for trypsin-inhibited studies
- as a component in radioimmunoprecipitation assay (RIPA) buffer to lyse leukocytes for western blot
Biochem/physiol Actions
Features and Benefits
- Broad specificity
- Non-toxic: After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.
- Contains no metal chelators: Ensures compatibility with downstream applications
- Convenient packaging: 1 mL in glass bottle
- Easy to use: Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.
Components
Bestatin
E-64
Leupeptin
Pepstatin A
Caution
Quantity
Physical form
Other Notes
related product
wgk_germany
WGK 1
flash_point_f
188.6 °F
flash_point_c
87 °C
Certificates of Analysis (COA)
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While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
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Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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