P5282

Phalloidin Peptide

≥90% (HPLC), solid, FITC labeled

• 동의어(들): Phalloidin-FITC
• 실험식(Hill 표기법): C₅₆H₆₀N₁₀O₁₅S₂
• Molecular Weight: 1177.26
• MDL number: MFCD00147902
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 제품명: Phalloidin, Fluorescein Isothiocyanate Labeled, sequence Amanita phalloides(synthetic: peptide sequence)
• 생물학적 소스: sequence from Amanita phalloides (synthetic: peptide sequence)
• Quality Level: 200
• 양식: solid
• 형광: λ_ex 495 nm; λ_em 520 nm(lit.)
• 저장 온도: −20°C

설명

일반 설명

Phalloidin is a phallotoxin produced by death cap mushroom Amanita phalloides. It is a cyclic peptide, which interacts with actin and this was first identified in phalloidin-poisoned rats. It is a heptapeptide, cyclic in nature, with a crosslink between tryptophan at position 6 and cysteine at position 3.[1] The side chain of amino acid 7 (γ-δ-dihydroxyleucine) in phalloidin, is accessible to modifications, through which fluorescently labelled phalloidin compounds can be produced.[2]

애플리케이션

Phalloidin, Fluorescein Isothiocyanate Labeled has been used:

• To visualize F-Actin reorganization in primary neonatal cardiomyocytes (PNCMs) and H9C2 cells (rat cardiac myoblasts) following endothelin-1 (ET-1) and angiotensin II (Ang II) treatment.[3]
• In immunochemistry to label microfilament.[4]
• In immunofluorescence analysis to stain F-actin.[5]

생화학적/생리학적 작용

Phalloidin interacts with polymeric actin, and not oligomeric or monomeric forms. This interaction leads to highly stabilized actin filaments, which resist depolymerization and disassembly. In rats, this toxin causes death due to liver hemorrhage, and cells show abnormal actin clustering.[1] The affinity of phalloidin to actin is not significantly altered after derivatizing fluorescently labelled phalloidin compounds. These compounds can be used to study actin structure and organization within eukaryotic cells.[2]

Toxin that binds polymeric F actin, stabilizing it and interfering with the function of actin-rich structures.

안전성 정보

• 픽토그램: GHS06
• 신호어: Danger
• 유해 및 위험 성명서: H300 + H310 + H330
• 예방조치 성명서: P260 - P262 - P264 - P280 - P302 + P352 + P310 - P304 + P340 + P310
• Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable