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Merck
모든 사진(1)

주요 문서

P7899

Sigma-Aldrich

Anti-Peroxidase antibody produced in rabbit

fractionated antiserum, lyophilized powder

동의어(들):

Rabbit Anti HRP

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.46
결합:
unconjugated
application:
ELISA (i)
클론:
polyclonal
종 반응성:
horseradish
citations:
36
기술:
indirect ELISA: 1:100,000-1:200,000

생물학적 소스

rabbit

Quality Level

결합

unconjugated

항체 형태

fractionated antiserum

항체 생산 유형

primary antibodies

클론

polyclonal

양식

lyophilized powder

종 반응성

horseradish

기술

indirect ELISA: 1:100,000-1:200,000

저장 온도

2-8°C

타겟 번역 후 변형

unmodified

일반 설명

Antiserum is determined to be immunospecific for horseradish peroxidase and does not inhibit the enzymatic activity of the peroxidase.
Peroxidase is a hemoprotein.
The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. If necessary, the fractionated antiserum is adsorbed using solid phase techniques. Antiserum is determined to be immunospecific by immunoelectrophoresis (IEP), against purified and crude horseradish peroxidase
The gel is stained with DAB (diaminobenzidine) to ensure that the antibody does not inhibit the enzymatic activity of the peroxidase. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion against anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation in the γ region
Each mL will precipitate a minimum of 0.25 mg of peroxidase at equivalence. May be used to stain axons and synaptic terminals in Drosophila embryo fillets.

면역원

Purified peroxidase from horseradish

애플리케이션

Anti-Peroxidase antibody produced in rabbit has been used in:
  • immunoblot assays
  • enzyme-linked-immunosorbent assay (ELISA)
  • immunoprecipitation

생화학적/생리학적 작용

Peroxidase helps in catalyzing the oxidation of a number of substrates with the help of hydrogen peroxide.

물리적 형태

Lyophilized from 0.01 M phosphate buffered saline, pH 7.2

재구성

Reconstitute with deionized water.

분석 메모

Specificity is determined by immunoelectrophoresis using a crude peroxidase extract.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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Lot/Batch Number

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문서 라이브러리 방문

Mary Lynn Dear et al.
Development (Cambridge, England), 143(1), 75-87 (2015-11-26)
Synaptogenesis requires orchestrated intercellular communication between synaptic partners, with trans-synaptic signals necessarily traversing the extracellular synaptomatrix separating presynaptic and postsynaptic cells. Extracellular matrix metalloproteinases (Mmps) regulated by secreted tissue inhibitors of metalloproteinases (Timps), cleave secreted and membrane-associated targets to sculpt
Koto Furotani et al.
PloS one, 13(9), e0204048-e0204048 (2018-09-19)
APP (amyloid precursor protein), the causative molecule of Alzheimer's disease, is synthesized in neuronal cell bodies and subsequently transported to synapses. We previously showed that the yata gene is required for the synaptic transport of the APP orthologue in Drosophila
Chilling injury and peroxidase activity changes in ?Fortune? mandarin fruit during low temperature storage
El-HF, et al.
Bulgarian Journal of Plant Physiology, 29(1-2), 44-54 (2003)
Bin Yang et al.
Oncology letters, 15(4), 4662-4668 (2018-03-16)
The present study aimed to assess whether different anesthesia methods (general anesthesia and general anesthesia combined with epidural block) were associated with tumor metastasis during the perioperative period and the possible molecular mechanisms of tumor metastasis. A rat hepatoma tumor
Tyrosine nitration of cytosolic peroxidase is probably triggered as a long distance signaling response in sunflower seedling cotyledons subjected to salt stress
Jain P and Bhatla SC
PLoS ONE, 13(5), e0197132-e0197132 (2018)

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