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A4416

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti Mouse Hrp Sigma
MDL number:
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

technique(s)

direct ELISA: 1:10,000

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
A8924A5278A3673
conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

technique(s)

direct ELISA: 1:10,000

technique(s)

direct ELISA: 1:10,000

technique(s)

direct ELISA: 1:10,000

technique(s)

direct ELISA: 1:15,000

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

species reactivity

mouse

species reactivity

mouse

species reactivity

-

species reactivity

mouse

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

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General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. Anti-Mouse IgG (whole molecule)-Peroxidase antibody is specific for mouse IgGs.

Immunogen

Purified mouse IgG

Application

Anti-Mouse IgG (whole molecule)-Peroxidase antibody is suitable for use in immunoblot (1:2000). The product can also be used for direct ELISA: 1:10,000.
Western blot analysis of transfected Hela cell lysates was performed using HRP conjugated goat anti-mouse IgG as the secondary at a dilution of 1:4000.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Y Wang et al.
The Journal of biological chemistry, 275(35), 27013-27020 (2000-06-17)
ATF6 is a member of the basic-leucine zipper family of transcription factors. It contains a transmembrane domain and is located in membranes of the endoplasmic reticulum. ATF6 has been implicated in the endoplasmic reticulum (ER) stress response pathway since it
F F Hsieh et al.
Blood, 96(8), 2746-2754 (2000-10-07)
Progression through the mammalian cell cycle is regulated by cyclins, cyclin- dependent kinases (CDKs), and cyclin-dependent kinase inhibitors (CKIs). The function of these proteins in the irreversible growth arrest associated with terminally differentiated cells is largely unknown. The function of
Jeffrey S Moffit et al.
The Journal of pharmacology and experimental therapeutics, 317(2), 537-545 (2006-02-10)
Hepatic transporters play a vital role in the disposition of endogenous compounds and xenobiotics in the liver. The current study investigates the expression and regulation of hepatic efflux transporters in response to treatment with the peroxisome proliferator-activated receptor (PPAR)alpha agonist
Takashi Umeyama et al.
Eukaryotic cell, 7(9), 1582-1590 (2008-07-16)
Trypanosoma brucei, the etiologic agent of African sleeping sickness, divides into insect (procyclic) and bloodstream forms. These two forms are subject to distinct cell cycle regulations, with cytokinesis controlled primarily by basal body/kinetoplast segregation in the procyclic form but by
Zhihai Mao et al.
PloS one, 8(7), e68206-e68206 (2013-07-23)
Metastasis remains to be one of the most prevalent causes leading to poor long-term survival of colorectal cancer (CRC) patients. The clinical significances of tumor metastatic suppressor, N-myc downregulated gene 1 (NDRG1), have been inconsistently reported in a variety of

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