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Goat Anti-Mouse IgG Antibody, HRP conjugate

Upstate®, from goat

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eCl@ss:
32160702
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

mouse

manufacturer/tradename

Upstate®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

shipped in

dry ice

target post-translational modification

unmodified

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This Item
AP181PAQ127AP181
vibrant-m

AQ127

Goat Anti-Mouse IgG Antibody, Fc

antibody form

purified immunoglobulin

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody form

F(ab′)2 fragment of affinity isolated antibody

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

unconjugated

conjugate

unconjugated

biological source

goat

biological source

goat

biological source

goat

biological source

goat

technique(s)

ELISA: suitable, western blot: suitable, immunohistochemistry: suitable

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

ELISA: suitable, immunoprecipitation (IP): suitable, western blot: suitable

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

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General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Specificity

Recognizes mouse IgG, both heavy and light chains.

Immunogen

Highly purified whole mouse IgG.

Application

Additional Research Applications
ELISA and Immunohistochemistry:
A working dilution of 1:10,000 to 1:40,000 is suggested for ELISA and 1:500 to 1:2,000 for immunohistochemistry.
Optimal antibody dilution for other applications should be determined by the researcher.
Goat Anti-Mouse IgG Antibody, HRP conjugate is an antibody against Mouse IgG for use in ELISA, IH & WB.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Quality

Western Blot Analysis:
Suitable for western blotting (dot blot) at 1:1,000 to 1:4,000 dilution.

Physical form

Purified goat polyclonal IgG in buffer containing conjugated to horseradish peroxidase lyophilized from 0.02 M Potassium Phosphate, 0.15 M NaCl, pH 7.2, 10 mg/mL BSA, and 0.01% gentamicin sulfate.
Rehydration: Add 500 µL sterile, distilled water containing 50% glycerol, to make a 1 mg/mL stock solution.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Research on promoting periodontal regeneration with human basic fibroblast growth factor-modified bone marrow mesenchymal stromal cell gene therapy.
Zhen Tan, Qing Zhao, Ping Gong, Yang Wu, Na Wei, Quan Yuan, Chuan Wang, Dapeng Liao, Hua Tang
Cytotherapy null
Ryan J Taft et al.
Epigenetics & chromatin, 4, 13-13 (2011-08-05)
Transcription initiation RNAs (tiRNAs) are nuclear localized 18 nucleotide RNAs derived from sequences immediately downstream of RNA polymerase II (RNAPII) transcription start sites. Previous reports have shown that tiRNAs are intimately correlated with gene expression, RNA polymerase II binding and
Swan Lin et al.
Pharmaceutical research, 33(1), 72-82 (2015-08-02)
To gain knowledge of lung clearance mechanisms of inhaled tissue plasminogen activator (tPA). Using an in vivo mouse model and ex vivo murine whole organ cell suspensions, we examined the capability of the lungs to utilize LRP1 receptor-mediated endocytosis (RME)
Jorid T Stuenaes et al.
British journal of pharmacology, 160(1), 116-129 (2010-04-24)
Genetic approaches have documented protein kinase B (PKB) as a pivotal regulator of heart function. Insulin strongly activates PKB, whereas adrenaline is not considered a major physiological regulator of PKB in heart. In skeletal muscles, however, adrenaline potentiates insulin-stimulated PKB
Yuanyuan Xiao et al.
Age (Dordrecht, Netherlands), 35(3), 573-582 (2012-01-31)
Exercise has been demonstrated to enhance subsequent insulin-stimulated glucose uptake (GU) by predominantly type II (fast-twitch) muscle of old rats, but previous research has not evaluated exercise effects on GU by type I (slow-twitch) muscle from old rats. Accordingly, we

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