P7937

Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit

5,000-15,000 units/mg protein, recombinant, expressed in E. coli, lyophilized powder

• 동의어(들): Protein phosphatase
• 효소 위원회 번호: 3.1.3.16 (BRENDA, IUBMB)
• MDL number: MFCD01635658
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 재조합: expressed in E. coli
• 분석: ≥80% (GE)
• 양식: lyophilized powder
• 특이 활성도: 5,000-15,000 units/mg protein
• 분자량: 37.5 kDa
• 기술: inhibition assay: suitable
• UniProt 수납 번호: P62139
• 저장 온도: −20°C
• 유전자 정보: rabbit ... PPP1CA(100009298)

설명

일반 설명

Protein phosphatase1 (PP1) is a heterodimeric enzyme with serine/threonine phosphatase activity. It comprises a catalytic subunit and a targeting subunit or a specific protein inhibitor. The targeting subunit determines the enzyme localization, e.g., to glycogen particles (subunit G) or to myofibrils (subunit M).

애플리케이션

Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit has been used as a standard in protein phosphatase-1 inhibition assay.

Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit has been used in protein phosphatase reaction and in the digestion of adipocyte extracts.

생화학적/생리학적 작용

Protein phosphatase1 (PP1) is involved in glycogen metabolism and in regulation of muscle contractility. In addition, it is implicated in cell cycle and transcriptional regulation. Recombinant rabbit PP1α has properties in common with the native rabbit muscle protein including size, requirement for Mn²⁺ for activity, specificity for phosphorylase A, and inhibition by okadaic acid, microcystin LR, and phosphatase inhibitor2 (I-2).

포장

Package size based on protein content.

단위 정의

One unit will hydrolyze 1 nmole of p-nitrophenyl phosphate per min at pH 7.4 at 30 °C.

물리적 형태

Lyophilized powder containing imidazole buffer, pH 7.4, NaCl, DTT, EDTA, MnCl₂, Tween 20, and trehalose as stabilizer.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)