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N4645

Sigma-Aldrich

4-Nitrophenyl phosphate disodium salt hexahydrate

powder, BioReagent, suitable for cell culture, ≥97%

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Synonym(s):
p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate
Linear Formula:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS Number:
Molecular Weight:
371.14
Beilstein/REAXYS Number:
5324661
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.75

biological source

synthetic (organic)

product line

BioReagent

assay

≥97%

form

powder

technique(s)

cell culture | mammalian: suitable

solubility

H2O: 100 mg/mL

storage temp.

−20°C

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

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1 of 4

This Item
N2640P5994SRE0026
vibrant-m

P5994

Phosphatase substrate

vibrant-m

SRE0026

Phosphatase substrate

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

-

assay

≥97%

assay

-

assay

-

assay

≥97% (HPLC)

solubility

H2O: 100 mg/mL

solubility

water: 1 tablet/3 mL, clear, colorless

solubility

H2O: soluble, clear, colorless to yellow (1 tablet in 8 mL of water.)

solubility

water: 100 mg/mL, clear to slightly hazy

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

form

powder

form

tablet

form

tablet

form

powder

General description

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Application

4-Nitrophenyl phosphate disodium salt hexahydrate has been used:
  • as a component in diethanolamine buffer to detect alkaline phosphatase-conjugated antibodies in enzyme linked immunosorbent assay (ELISA)
  • to treat adherent cells in adhesion assay
  • as a phosphatase substrate in in vitro phosphatase assay in substrate buffer for ELISA

Biochem/physiol Actions

p-Nitrophenyl phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in enzyme linked immunosorbent assay (ELISA) procedures. It is the substrate of choice in alkaline phosphatase systems due to its high sensitivity. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. This product is tested for use in cell culture applications. 4-nitrophenyl phosphate (pNPP), a small-molecule substrate can be used to study on alkaline phosphatases, acid phosphatases etc.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Chart H, et al.
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