S0692

Spectinomycin

Ready Made Solution, 100 mg/mL in DMSO/H₂O, 1:1

• 동의어(들): Actinospectacin
• MDL number: MFCD04041129
• UNSPSC 코드: 51281602
• NACRES: NA.85

속성

• 생물학적 소스: Streptomyces sp.
• Quality Level: 200
• 형태: solution
• 농도: 100 mg/mL in DMSO/H₂O, 1:1
• 색상: colorless
• solubility: DMSO/H₂O, 1:1: soluble 100 mg/mL
• 항생제 활성 스펙트럼: Gram-negative bacteria; Gram-positive bacteria; mycobacteria
• 동작 모드: protein synthesis | interferes
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

일반 설명

Chemical structure: aminoglycoside

애플리케이션

Spectinomycin is an aminocyclitol antibiotic derived from Streptomyces spectabilis. It has been shown to have a wide variety of uses, including treating acute gonorrheal urethritis and cervictis, to mark cell layers to monitor cell fate during leaf development, as a selection marker in plant related transformation systems for plant cells containing the marker gene Spcr, to study respiratory tract infections of cattle caused by Pasteurella multocida and Mannheimia haemolytica, and to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media. Spectinomycin also has use in amplification of low copy number plasmid carrying replicons. It is recommended for use in cell culture applications at 7.5-20 mg/L.

생화학적/생리학적 작용

Mode of Action: Spectinomycin acts by inhibiting protein synthesis and elongation by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation.

Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3′-adenyltransferase gene (aadA).

Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria.

주의사항

Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods.

제조 메모

Stock solutions should be prepared at 10 mg/mL in water and 0.2 μm filtered.

저장 및 안정성

Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.Store under inert gas.

기타 정보

Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage. Store under inert gas

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): 201.0 °F - closed cup
• Flash Point (°C): 93.9 °C - closed cup
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)