S3401

Sample Buffer, Laemmli 2× Concentrate

• 동의어(들): SDS Sample Buffer
• UNSPSC 코드: 41105323
• NACRES: NA.25

속성

• 포장: vial of 6 mL
• Quality Level: 200
• 저장 온도: −20°C

설명

애플리케이션

Sample Buffer, Laemmli 2× concentrate has been used as a sample buffer for denaturing and loading of protein samples in SDS-PAGE.

생화학적/생리학적 작용

Laemmli sample buffer is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min. The 2-mercaptoethanol reduces the intra and inter-molecular disulfide bonds. The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. The glycerol increases the density of the sample so that it will layer in the sample well.

기타 정보

Solution contains 4% SDS, 20% glycerol, 10% 2-mercaptoethanol, 0.004% bromphenol blue and 0.125 M Tris HCl, pH approx. 6.8.

수량

Recommended usage: add 1 volume gel loading solution to 1 volume sample and mix well.

안전성 정보

• 픽토그램: GHS05,GHS06,GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H302 - H311 - H315 - H317 - H318 - H361d - H373 - H412
• 예방조치 성명서: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter