S5313

Anti-Sir2 (AS-16) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• 동의어(들): Anti-SIR2, Anti-SIR2L1, Anti-SIR2alpha
• MDL number: MFCD03792039
• UNSPSC 코드: 12352203
• NACRES: NA.41
• 결합: unconjugated
• application: ARR; IF; WB
• 클론: polyclonal
• 종 반응성: mouse
• citations: 9
• 기술: indirect immunofluorescence: 1:50 using mouse NIH3T3 cells; microarray: suitable; western blot: 1:2,000 using mouse NIH/3T3 cells

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: buffered aqueous solution
• 분자량: antigen ~110 kDa
• 종 반응성: mouse
• 포장: antibody small pack of 25 μL
• 기술: indirect immunofluorescence: 1:50 using mouse NIH3T3 cells; microarray: suitable; western blot: 1:2,000 using mouse NIH/3T3 cells
• UniProt 수납 번호: Q923E4
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Sirt1(93759)

설명

일반 설명

Sir2 belongs to a family of proteins that is found in organisms ranging from bacteria to complex eukaryotes. Members of this family contain a 250 amino acid core domain that shares about 25-60% sequence identity.

특이성

Anti-Sir2 polyclonal antibody specifically recognizes mouse Sir2 (AS-16). Staining of the Sir2 band is specifically inhibited by the immunizing peptide.

면역원

synthetic peptide corresponding to mouse Sir2 sequence (amino acids 722-737 with N-terminal added lysine), conjugated to KLH. This sequence is 62% homologous to the corresponding human sequence.

애플리케이션

Anti-Sir2 (AS-16) antibody produced in rabbit has been used in

• western blotting
• immunoprecipitation
• antibody microarray

생화학적/생리학적 작용

Sir2, one of the silent information regulator genes, encodes a protein that promotes a compact chromatin structure, thereby preventing or silencing gene transcription at selected loci. Silencing occurs as a series of events initiated by formation of Sir complexes (Sir2, Sir3, Sir4). Sir2 protein is an NAD-dependent histone deacetylase. Sir2 transfers acetyl groups from its protein substrates to ADP-ribose and synthesizes o-acetyl ADP-ribose. Through histone deacetylation, Sir2 may silence chromatin. It appears that Sir2 NAD requirement makes this protein an important player in the pathway that leads to increased life span of several species through calorie restriction.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable