추천 제품
분석
≥90% (HPLC)
형태
ready-to-use solution
저장 조건
protect from light
배송 상태
dry ice
저장 온도
−20°C
일반 설명
Filipin Complex, the collective name given to family of isomeric polyene macrolides isolated from cultures of S. filipinensis, with Filipin III being the majority part of the complex.
애플리케이션
Filipin complex readymade solution has been used in the staining of HeLa cells for cytoskeletal imaging.
Filipin has been used in a double staining procedure as a probe for the detection of lipoproteins in polyacrylamide gel and immobilized on nitrocellulose membranes. It is also widely used to localize and quantitate unesterified cholesterol by virtue of a specific fluorescent complex.
생화학적/생리학적 작용
Filipin is an antibiotic with antifungal functionality. Filipin complex has been found to alter cell membrane structure by interacting with the membrane sterols ergosterols and cholesterol. On binding to cholesterol, filipin shows fluorescence shift. As such, filipin complex has become a useful tool for diagnosis of Niemann-Pick type C disease, and for detection and quantification of cholesterol in cell membranes and lipid quantification in brain.
기타 정보
Filipin complex ready made solution is very sensitive to air and light. Upon receipt aliquot and store at -20°C. Avoid freeze thaw cycles.
Filipin complex is used in various concentrations depending on the specific protocol used. Dilute 5mg/ml stock solution with appropriate buffer according to the used protocol.
Filipin complex interaction with cholesterol alters the absorption and fluorescence spectra, for visualization with a fluorescence microscope use excitation at 340-380 nm and emission at 385-470 nm (Filipin fluorescent staining photo-bleaches very rapidly, thus the sample should be analyzed immediately).
Filipin complex is used in various concentrations depending on the specific protocol used. Dilute 5mg/ml stock solution with appropriate buffer according to the used protocol.
Filipin complex interaction with cholesterol alters the absorption and fluorescence spectra, for visualization with a fluorescence microscope use excitation at 340-380 nm and emission at 385-470 nm (Filipin fluorescent staining photo-bleaches very rapidly, thus the sample should be analyzed immediately).
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
188.6 °F - closed cup
Flash Point (°C)
87 °C - closed cup
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
The pentaene macrolide antibiotic filipin prefers more rigid DPPC bilayers: a fluorescence pressure dependence study
Castanho MRB, et al.
Biochimica et Biophysica Acta - Biomembranes, 1419(1), 1-14 (1999)
Xi Zhao et al.
Redox biology, 62, 102678-102678 (2023-03-21)
Elevated lipid peroxidation (LPO), usually present in the tumour microenvironment (TME), is profoundly implicated in antitumour immunity and may be targeted for the development of new antitumour therapies. However, tumour cells may also rewire their metabolism to survive elevated LPO.
Filipin recognizes both GM1 and cholesterol in GM1 gangliosidosis mouse brain
Arthur JR, et al.
Journal of Lipid Research, 52(7), 1345-1351 (2011)
Maximilian Jobst et al.
Archives of toxicology (2022-10-11)
Bladder cells are constantly exposed to multiple xenobiotics and bioactive metabolites. In addition to this challenging chemical environment, they are also exposed to shear stress originating from urine and interstitial fluids. Hence, physiological function of bladder cells relies on a
Liyang Li et al.
Clinical and translational medicine, 12(6), e902-e902 (2022-06-10)
Hypercholesterolemia is found in patients with chronic lung inflammation, during which airway epithelial cells play important roles in maintenance of inflammatory responses to pathogens. The present study aims at molecular mechanisms by which cholesterol changes airway epithelial sensitivity in response
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