11191

Histopaque^®-1119

sterile-filtered, density: 1.119

• NACRES: NA.75
• UNSPSC Code: 12352207

คุณสมบัติ

• sterility: sterile-filtered
• Quality Level: 400
• form: liquid
• shelf life: Expiry date on the label.
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• density: 1.119
• application(s): hematology; histology
• storage temp.: 2-8°C

คำอธิบาย

General description

Histopaque^®-1119 is a sterile, endotoxin tested, ready-to-use medium. This solution consists of polysucrose and sodium diatrizoate, adjusted to a relative density of 1.119 g/mL and pH 8.8–9.0.

Application

Histopaque^®-1119 is suitable for use in conjunction with Histopaque-1077 as it facilitates the rapid recovery of viable mononuclear cells and granulocytes from small volumes of whole blood or bone marrow

Biochem/physiol Actions

The process involves the formation of a double gradient on layering an equal volume of Histopaque-1077 over Histopaque-1119. Anticoagulated whole blood is carefully layered onto the upper Histopaque-1077 medium. Upon centrifugation, the erythrocytes are aggregated by polysucrose and thus rapidly sediment. Granulocytes are found at the lower Histopaque-1077/1119 interface; whereas, lymphocytes and other mononuclear cells are found at the upper plasma/Histopaque-1077 interface. Most extraneous platelets found in the upper interface are removed by low-speed centrifugation during washing steps. Erythrocyte contamination is negligible.

Features and Benefits

Histopaque^®-1119 offers numerous advantages, such as:

• Optimal recovery of viable cells
• Permits selective separation of blood cell types
• Prevents cell distortion
• Preserves cell viability
• Minimal extraneous cell interference
• Reproducible performance from batch to batch
• Sterile-filtered and endotoxin-tested
• Stable at 2-8°C for at least 2 years when protected from light

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

ข้อมูลความปลอดภัย

• pictograms: GHS05
• signalword: Warning
• hcodes: H290,H315,H319
• pcodes: P234 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Hazard Classifications: Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• คลาสการจัดเก็บ: 10 - Combustible liquids