NA0300S
GenElute™ HP Plasmid Maxiprep Kit
sufficient for 4 purifications
Synonym(s):
GenElute™ HP Plasmid Maxiprep Kit
เข้าสู่ระบบ เพื่อดูราคาสำหรับองค์กรและสัญญา
เลือกขนาด
เปลี่ยนการดู
เกี่ยวกับสินค้านี้
NACRES:
NA.52
UNSPSC Code:
41105501
บริการทางเทคนิค
ต้องการความช่วยเหลือหรือไม่ ทีมนักวิทยาศาสตร์ที่มีประสบการณ์ของเราอยู่ที่นี่เพื่อคุณ
ให้เราช่วยเหลือusage
sufficient for 4 purifications
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
DNA purification: suitable
test parameters
sample volume: 150 mL bacterial culture
greener alternative category
storage temp.
15-25°C
General description
The GenElute HP Plasmid Maxiprep Kit offers a simple, rapid, and cost effective method for isolating plasmid DNA from recombinant E. coli cultures. The kit features a filter syringe for the rapid clearing of lysate and a silica binding column designed for either a vacuum or a spin format. Up to 1.2 mg of plasmid DNA can be isolated from a 150 mL overnight culture grown in Luria Broth (LB) medium. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.
An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto a silica membrane in the presence of high salts. Contaminants are removed by two wash steps. Finally, the bound DNA is eluted in Elution Solution (Tris-HCl) or water.
The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection.
An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto a silica membrane in the presence of high salts. Contaminants are removed by two wash steps. Finally, the bound DNA is eluted in Elution Solution (Tris-HCl) or water.
The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Application
GenElute™ HP Plasmid Maxiprep Kit has been used to isolate plasmid DNA from Escherichia coli.
Features and Benefits
- From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
- Up to 1.2 mg of high-copy plasmid DNA
- Flexibility of a vacuum or spin format
- Contains fewer plastic components than other high speed kits, reducing the amount of waste
- No phenol/chloroform extraction or alcohol precipitation required
Other Notes
For additional information, please see www.sigma-aldrich.com/genelutehp.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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signalword
Danger
target_organs
Central nervous system
คลาสการจัดเก็บ
3 - Flammable liquids
flash_point_f
77.0 °F - closed cup
flash_point_c
25 °C - closed cup
wgk
WGK 3
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
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โปรโตคอล
Follow this simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures with the GenElute™ HP Plasmid Maxiprep kit.
Lisa A Santry et al.
Molecular therapy. Methods & clinical development, 9, 181-191 (2018-03-21)
Newcastle disease virus (NDV) is a single-stranded, negative-sense RNA virus in the Paramyxoviridae family. Although primarily an avian pathogen, NDV is a potent oncolytic virus that has been shown to be safe and effective in a variety of preclinical cancer
David A Rasko et al.
Plasmid, 58(2), 159-166 (2007-04-27)
Francisella tularensis is a category A bioterror pathogen which in some cases can cause a severe and fatal human infection. Very few virulence factors are known in this species due to the difficulty in working with it as well as
Development of novel plasmid vectors and a promoter trap system in Francisella tularensis compatible with the pFLN10 based plasmids
David A Rasko
Plasmid, 58(2) (2007)