P0044
Phosphatase Inhibitor Cocktail 3
DMSO solution, for the inhibition of protein phosphatase 2A, alkaline phosphatases, protein phosphatases 1 and 2A, DMSO solution
Synonym(s):
Phosphatase inhibitor
เข้าสู่ระบบ เพื่อดูราคาสำหรับองค์กรและสัญญา
เลือกขนาด
เปลี่ยนการดู
เกี่ยวกับสินค้านี้
UNSPSC Code:
12352200
NACRES:
NA.77
EC Number:
200-664-3
Form:
DMSO solution
Solubility:
water: soluble
Storage temp.:
2-8°C
บริการทางเทคนิค
ต้องการความช่วยเหลือหรือไม่ ทีมนักวิทยาศาสตร์ที่มีประสบการณ์ของเราอยู่ที่นี่เพื่อคุณ
ให้เราช่วยเหลือชื่อสินค้า
Phosphatase Inhibitor Cocktail 3, DMSO solution
form
DMSO solution
Quality Level
solubility
water: soluble
shipped in
wet ice
storage temp.
2-8°C
Application
The phosphatase inhibitor cocktail improves the yields of intact proteins by adding inhibitors to enzymes that modify proteins present in cell extracts. This product has been tested on extract from animal tissues including human placenta and bovine liver, and on extracts of A431, CHO, and U937 cells. It has been optimized and tested for L-isozymes of alkaline phosphatase as measured with pNPP and for serine-threonine protein phosphatases.
Biochem/physiol Actions
This mixture contains individual components with specific inhibitory properties. Cantharidin inhibits protein phosphatase 2A. (-)-p-Bromolevamisole oxalate inhibits L-isoforms of alkaline phosphatases. Calyculin A inhibits protein phosphatases 1 and 2A.
Preparation Note
This cocktail is supplied as a clear solution in dimethyl sulfoxide. It is recommended to dilute the cocktail at 1 mL of cocktail per 500 mg of protein extracted from tissue or cells. Often a final concentration of 1% (v/v) is used, which will inhibit phosphatase activities found in the 27,000 x g supernatant from human placenta and bovine liver, and in the 100,000 x g supernatant from A431, CHO, and U937 extracts.
Disclaimer
As supplied, this product is stable for four years at the recommended 2-8°C storage temperature.
คลาสการจัดเก็บ
10 - Combustible liquids
flash_point_f
188.6 °F
flash_point_c
87 °C
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บทความ
The use of phosphatase inhibitors is critical for all types of phosphorylation studies. Compare phosphatase inhibitor cocktails to select the best one for your research.
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