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PTRP24H48

Millipore

Millicell® 24 well hanging cell culture inserts

pore size 1.0 μm, clear PET membrane, diam. 6.5 mm, Tissue Culture (TC)-treated surface, sterile

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Synonym(s):
cell culture inserts, permeable culture insert, plate inserts, tissue culture insert, tissue culture plate

material

clear PET membrane
polystyrene housing

Quality Level

sterility

γ-irradiated
sterile

feature

2x106 pores/cm2

packaging

pack of 48 ea (individually blister packaged)

parameter

37 °C max. temp.

technique(s)

cell attachment: suitable
cell differentiation: suitable
drug transporter assay: suitable
immunocytochemistry: suitable
permeability assay: suitable

H

15.5 mm

diam.

6.5 mm

insert maximum volume

0.4 mL

insert working volume

0.2 mL

size

24 wells

surface area

0.3 cm2

color

clear membrane
transparent membrane

pore size

1.0 μm pore size

binding type

Tissue Culture (TC)-treated surface

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This Item
PTMP24H48PTHT24H48PTEP24H48
sterility

γ-irradiated, sterile

sterility

γ-irradiated, sterile

sterility

γ-irradiated, sterile

sterility

γ-irradiated, sterile

feature

2x106 pores/cm2

feature

0.6x106 pores/cm2

feature

100x106 pores/cm2

feature

0.2x106 pores/cm2

packaging

pack of 48 ea (individually blister packaged)

packaging

pack of 48 ea (individually blister packaged)

packaging

pack of 48 ea (individually blister packaged)

packaging

pack of 48 ea (individually blister packaged)

size

24 wells

size

24 wells

size

24 wells

size

24 wells

pore size

1.0 μm pore size

pore size

5.0 μm

pore size

0.4 μm

pore size

8.0 μm pore size

General description

Millicell® Cell Culture inserts with Polyethylene Terephthalate (PET) membrane are ideal for the growth and differentiation of various cell types. This individually sterile-packaged insert has an optically clear 1.0 μm membrane pore size and fits in most 24-well tissue culture plates.

In tissue culture plates, cells can access media only from their apical sides, but in Millicell® tissue culture inserts, cells can access media from both their apical and basolateral sides. As result, cell growth, structure, and function more closely mimic what occurs in vivo.

The insert’s flanged design suspends it within the culture plate well and makes it possible to access both sides of the cell monolayer. Spaces between three protruding arms facilitate pipetting between the outside of the insert and the culture plate well.

Application

  • Cell Proliferation
  • Cell Surface Receptors
  • Co-Culture
  • Epithelial Cell Growth
  • Feeder Layers
  • Fluorescent Detection /Immunohistochemistry
  • In Vitro Fertilization
  • In Vitro Toxicology
  • Microbial Attachment
  • Phase Contrast Microscopy
  • Polarized Protein Secretions
  • Polarized Uptake
  • Transport/Permeability

Specifications

Membrane pore density = 2 x 106 pores/cm2

Legal Information

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articles

How to set up barrier formation and permeability assays using cell culture inserts and TEER measurements.

Millicell® hanging cell culture inserts for staining and microscopic analysis directly from the insert. Learn to utilize hanging cell culture inserts in barrier measurements.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service