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EAGLETMMRO

Roche

EagleTaq Universal Master Mix (ROX)

sufficient for 100 reactions, sufficient for 500 reactions, sufficient for 5000 reactions, suitable for qPCR, suitable for RT-qPCR

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usage

sufficient for 100 reactions
sufficient for 500 reactions
sufficient for 5000 reactions

Quality Level

feature

dNTPs included
hotstart

manufacturer/tradename

Roche

packaging

pkg of 100 x 20 μL reactions (07260288190)
pkg of 500 x 20 μL reactions (07260296190)
pkg of 5000 x 20 μL reactions (07249926190)

technique(s)

RT-qPCR: suitable
qPCR: suitable

input

purified DNA

detection method

probe-based

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P4600KCQS09FSTMPMMRO
ReadyMix™ Taq PCR Reaction Mix with MgCl2

P4600

ReadyMix Taq PCR Reaction Mix

FastStart TaqMan® Probe Master sufficient for 100 reactions, sufficient for 500 reactions, sufficient for 2000 reactions, suitable for qPCR, suitable for RT-qPCR

FSTMPMMRO

FastStart TaqMan® Probe Master

usage

sufficient for 100 reactions

usage

sufficient for 100 reactions

usage

sufficient for 500 reactions

usage

sufficient for 100 reactions, sufficient for 2000 reactions, sufficient for 500 reactions

feature

dNTPs included

feature

dNTPs included, hotstart: no

feature

dNTPs included, hotstart

feature

dNTPs included: no, hotstart

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

Roche

packaging

pkg of 100 x 20 μL reactions (07260288190)

packaging

-

packaging

-

packaging

pkg of 100 x 50 μL reactions (04673409001), pkg of 2000 x 50 μL reactions (04673433001), pkg of 500 x 50 μL reactions (04673417001)

input

purified DNA

input

purified DNA

input

purified RNA

input

purified DNA

General description

2× concentrated, ready-to-use hot start master mix for qPCR and qRT-PCR using the hydrolysis probe detection format on real-time PCR instruments.

The EagleTaq Universal Master Mix (ROX) is a ready-to-use, 2× concentrated PCR master mix that contains all the reagents (except primers, probes, and template) needed for performing quantitative, real-time PCR hydrolysis probe reactions. It contains a special ROX reference dye which makes it suitable for all real-time instruments on which a ROX reference dye is needed for quantitative analysis.
The EagleTaq Universal Master Mix (ROX) contains dUTP so that it may be used with Uracil-DNA Glycosylase (UNG) to prevent false positives arising from carryover contamination.
The hot start properties allow reaction setup at ambient temperature.
With this robust reagent, any PCR protocol optimization is minimized
The EagleTaq Universal Master Mix (ROX) is a ready-to-use, 2× concentrated polymerase chain reaction (PCR) master mix that contains all the reagents (except primers, probes, and template) needed for performing quantitative, real-time PCR (qPCR) and quantitative reverse transcription PCR (qRT-PCR) hydrolysis probe reactions.

Application

EagleTaq Universal Master Mix (ROX) has been used in quantitative, real-time polymerase chain reaction (qPCR ) and quantitative reverse transcription PCR (qRT-PCR).

Features and Benefits

  • The EagleTaq Universal Master Mix (ROX) contains a special ROX reference dye which makes it suitable for all real-time instruments on which a ROX reference dye is needed for quantitative analysis.
  • It contains dUTP so that it may be used with Uracil-DNA Glycosylase (UNG) to prevent false positives arising from carryover contamination.
  • The hot start properties allow reaction setup at ambient temperature.
  • With this robust reagent, any PCR protocol optimization is minimized.

Components

Contains EagleTaq DNA Polymerase and a reaction buffer with dATP, dCTP, dGTP, dUTP, MgCl2, and ROX.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


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PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.

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Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.

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