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12306C

Sigma-Aldrich

Fetal Bovine Serum

non-USA origin, from USDA approved countries, Heat Inactivated, sterile-filtered, suitable for cell culture

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Synonym(s):
HI FBS, HI FCS, HI Fetal Bovine Serum, HI Fetal Calf Sera, Heat Inactivated Fetal Calf Sera, FBS, FCS, sera, serum
MDL number:
MFCD00132239
NACRES:
NA.71

biological source

bovine fetus

Quality Level

500

sterility

sterile-filtered

product line

FBS Premier

quality

from USDA approved countries

composition

Hemoglobin, ≤25 mg/dL

origin

non-USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

storage temp.

−20°C

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Fetal Bovine Serum non-USA origin, from USDA approved countries, Heat Inactivated, sterile-filtered, suitable for cell culture

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12306C

Fetal Bovine Serum

Fetal Bovine Serum USA origin, Charcoal Stripped, sterile-filtered, suitable for cell culture

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Fetal Bovine Serum

Fetal Bovine Serum Australia origin, USDA approved, sterile-filtered, suitable for cell culture

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12003C

Fetal Bovine Serum

Fetal Bovine Serum Australia origin, suitable for, USDA approved, sterile-filtered, suitable for cell culture, suitable for hybridoma

Sigma-Aldrich

F9423

Fetal Bovine Serum

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

quality

from USDA approved countries

quality

-

quality

USDA approved

quality

USDA approved

composition

Hemoglobin, ≤25 mg/dL

composition

Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL

composition

Hemoglobin, ≤25 mg/dL

composition

Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL

origin

non-USA origin

origin

USA origin

origin

Australia origin

origin

Australia origin

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

Application

FBS sourced from USDA approved non-USA countries is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.
Fetal Bovine Serum has been used to block the human induced pluripotent stem cells (hiPSCs) derived hepatocyte-like cells (HLCs) cells in phosphate buffered saline during immunofluorescence staining.

Biochem/physiol Actions

Fetal Bovine Serum (FBS) is used as a supplement to cell culture media. It provides many non-defined growth promoting and survival enhancing factors to cells in culture.

Suitability

USDA Approved Sourcing refers to FBS produced from blood collected in countries outside of the United States that have been approved by the USDA (United States Department of Agriculture) for export of beef products into the United States. Eligible countries for export into the U.S. include Australia, Canada, Chile, Costa Rica, Honduras, Iceland, Japan, Mexico, New Zealand, Nicaragua, Uruguay. Foreign serum is tested to ensure the absence of exotic viruses before release by the USDA.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Differentiation and transplantation of human induced pluripotent stem cell-derived hepatocyte-like cells
Asgari S, et al.
Stem Cell Reviews and Reports, 9(4), 493-504 (2013)
Generation of functional hepatocyte-like cells from human pluripotent stem cells in a scalable suspension culture
Vosough M, et al.
Stem Cells and Development, 22(20), 2693-2705 (2013)
Shao Thing Teoh et al.
Cancer letters, 492, 21-30 (2020-08-10)
Dysregulated metabolism is a hallmark of cancer that supports tumor growth and metastasis. One understudied aspect of cancer metabolism is altered nucleotide sugar biosynthesis, which drives aberrant cell surface glycosylation known to support various aspects of cancer cell behavior including
Xiao-Min Liu et al.
Nature chemical biology, 15(9), 865-871 (2019-08-07)
RNA modification in the form of N6-methyladenosine (m6A) regulates nearly all the post-transcriptional processes. The asymmetric m6A deposition suggests that regional methylation may have distinct functional consequences. However, current RNA biology tools do not distinguish the contribution of individual m6A
Gerhard Gstraunthaler
ALTEX, 20(4), 275-281 (2003-12-13)
Serum is commonly used as a supplement to cell culture media. It provides a broad spectrum of macromolecules, carrier proteins for lipoid substances and trace elements, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors. The
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