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69890

Sigma-Aldrich

MES hydrate

BioUltra, ≥99.5% (T)

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Synonym(s):
2-(N-Morpholino)ethanesulfonic acid hydrate, 4-Morpholineethanesulfonic acid
Empirical Formula (Hill Notation):
C6H13NO4S · xH2O
CAS Number:
Molecular Weight:
195.24 (anhydrous basis)
MDL number:
eCl@ss:
32129211
PubChem Substance ID:

product line

BioUltra

Assay

≥99.5% (T)

form

powder or crystals

impurities

insoluble matter, passes filter test

ign. residue (900 °C)

≤0.05%

pH

2.5-4.0 (25 °C, 0.5 M in H2O)

useful pH range

5.5-6.7

pKa 

6.1

solubility

H2O: 0.5 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤100 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤20 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

0.5 M in H2O

UV absorption

λ: 260 nm Amax: 0.025
λ: 280 nm Amax: 0.020

SMILES string

O.OS(=O)(=O)CCN1CCOCC1

InChI

1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2

InChI key

MIIIXQJBDGSIKL-UHFFFAOYSA-N

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This Item
M5287M8250M2933
MES hydrate BioUltra, ≥99.5% (T)

Sigma-Aldrich

69890

MES hydrate

Premium Grade
MES hydrate ≥99.5% (titration), pH 2.5-4.0 (0.5 M in H2O), BioXtra

Sigma-Aldrich

M5287

MES hydrate

Premium Grade
MES hydrate ≥99.5% (titration)

Sigma-Aldrich

M8250

MES hydrate

-
MES hydrate BioPerformance Certified, suitable for cell culture, ≥99.5%

Sigma-Aldrich

M2933

MES hydrate

Premium Grade
assay

≥99.5% (T)

assay

≥99.5% (titration)

assay

≥99.5% (titration)

assay

≥99.5%

form

powder or crystals

form

crystalline powder

form

crystalline powder

form

crystalline powder

impurities

insoluble matter, passes filter test

impurities

Insoluble matter, passes filter test

impurities

-

impurities

endotoxin and Total Aerobic Microbial Count, tested

pH

2.5-4.0 (25 °C, 0.5 M in H2O)

pH

2.5-4.0 (0.5 M in H2O)

pH

-

pH

-

solubility

H2O: 0.5 M at 20 °C, clear, colorless

solubility

H2O: 0.5 M, clear, colorless

solubility

water: 0.25 g/mL, clear, colorless

solubility

-

General description

MES is one of the "Good" buffers developed for biological applications. MES is not recommended at pH 7.4; other buffers should be considered. All qualities are tested for trace ions and all can be used for biological and biochemistry applications. This product is used in biochemical and biological techniques where highly purified products are needed.

Preparation Note

A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa=±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.

Other Notes

Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8°C for months.
Sterilization: Sterilization should be by filteration through 0.2μM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unknown.
Easily compare specifications for MES hydrate products with the MES hydrate specification table.
Biological buffer; useful pH-range 5.8-6.5; Mobile phase component in brain tubulin isolation by amino-activated gel chromatography; Buffer in plant tissue culture.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

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  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

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D.E. Parfitt
Sci. Hortic., 36, 157-157 (1988)
E Lacey et al.
Journal of chromatography, 525(1), 71-84 (1990-01-26)
In the present study, we report the isolation of the acidic structural protein tubulin using a number of amino-activated gels. Crude 100,000 g supernatant derived from sheep brain was applied to gels activated with either aminohexyl, aminoethyl, argininyl, diethylaminoethyl, lysinyl
M De Block
Plant physiology, 93(3), 1110-1116 (1990-07-01)
Tissue culture conditions and transformation have been established for both aspen and poplar. The use of previously described culture conditions resulted in shoot tip necrosis in the shoot cultures and necrosis of stem and leaf explants. Shoot tip necrosis could
Dawson, R.M.C. et al.
Data for Biochemical Research, 410- 410 (1987)
Good NE et al.
Biochemistry, 5, 467-477 (1966)

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