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94046

Sigma-Aldrich

Sodium phosphate dibasic solution

BioUltra, 0.5 M in H2O

Synonym(s):

Disodium hydrogen phosphate solution, di-Sodium hydrogen phosphate solution

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About This Item

Linear Formula:
Na2HPO4
CAS Number:
7558-79-4
Molecular Weight:
141.96
MDL number:
MFCD00003496
UNSPSC Code:
12161700
eCl@ss:
38070211
PubChem Substance ID:
57653505
NACRES:
NA.25

product line

BioUltra

Quality Level

100

form

liquid

concentration

0.5 M in H2O

impurities

insoluble matter, passes filter test
≤0.001% total nitrogen (N)

pH

8.8-9.4 (25 °C, 0.5 M in H2O)

solubility

H2O: 0.5 M at 20 °C, clear, colorless

density

1.064 g/mL at 20 °C

anion traces

chloride (Cl-): ≤10 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

0.5 M in H2O

UV absorption

λ: 260 nm Amax: 0.019
λ: 280 nm Amax: 0.016

application(s)

diagnostic assay manufacturing

SMILES string

[Na+].[Na+].OP([O-])([O-])=O

InChI

1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2

InChI key

BNIILDVGGAEEIG-UHFFFAOYSA-L

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Application

Sodium phosphate dibasic solution has been used:
  • to supplement Dulbecco′s Modified Eagle Medium (DMEM) in calcifying medium to induce the calcification of vascular smooth muscle cells (VSMCs)
  • to prepare a stock solution of CaCl2 and MgCl2 to synthesize calcium phosphate (CPB) and magnesium phosphate bions (MPBs) respectively
  • to supplement 2xYT agar plates to culture BL21 DE3 star cells and BL21dLac Gold cells

Sodium phosphate dibasic solution may be used in the preparation of a wide variety of phosphate based biological buffers for use in cell and molecular biology application.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Filis Kazazi-Hyseni et al.
Pharmaceutical research, 31(10), 2844-2856 (2014-05-16)
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Protocols

Enzymatic Assay of Alcohol Dehydrogenase (EC 1.1.1.1)

To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

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