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D7295

Sigma-Aldrich

Deoxynucleotide Mix, 10 mM

Molecular Biology Reagent

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Synonym(s):
10mM dNTP mix, dNTP mix, dNTPs
NACRES:
NA.52

Quality Level

form

liquid

concentration

10 mM

color

colorless

application(s)

agriculture

foreign activity

DNase, RNase, none detected

shipped in

dry ice

storage temp.

−20°C

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This Item
DNTP10DNTP100AD6920
concentration

10 mM

concentration

10 mM

concentration

100 mM

concentration

10 mM

color

colorless

color

colorless

color

colorless

color

colorless

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

300

Quality Level

200

Quality Level

300

Quality Level

200

General description

Nucleotides are organic molecules that serve as the monomers, or subunits, of nucleic acids (like DNA and RNA). The building blocks of nucleic acids, nucleotides consist of a nitrogenous base (purine or pyrimidine), a five-carbon sugar (ribose or deoxyribose), and at least one phosphate group. A nucleoside along with a phosphate group yields a nucleotide. The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM each of UltraPure dATP, dCTP, dGTP, and TTP sodium salts in high-quality molecular biology grade water. One µL is sufficient for a standard 50 µL PCR reaction.

Application

dNTP Mix has been used:

  • in the PCR amplification of genomic DNA isolated from insect, fungi, virus, human,
  • in reverse transcription of total RNA to cDNA.
  • as a component of the DNA amplification mixture for polymerase chain reaction (PCR)
  • routine and long PCR
  • manual and automated DNA sequencing
  • cDNA synthesis and labeling reactions

Features and Benefits

  • Purity of each dNTP: Minimum 99%
  • Conveniently formulated; 1 μL is used per 50 μL PCR
  • Equimolar amounts of each dNTP means less pipetting
  • Minimize risk of contamination in PCR
  • UltraPure dNTPs can help maximize consistency and yields in critical PCR reactions

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Protocols

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.

Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA.

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