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D8045

Sigma-Aldrich

AccuTaq LA DNA Polymerase

High fidelity Taq enzyme, with 10X buffer & DMSO

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Synonym(s):
High Fidelity DNA polymerase, High fidelity PCR, Long range PCR
MDL number:
NACRES:
NA.55

Quality Level

form

liquid

usage

sufficient for 125 reactions
sufficient for 1500 reactions
sufficient for 500 reactions

feature

Long & Accurate PCR
dNTPs included: no
hotstart: no

concentration

5 units/μL

technique(s)

PCR: suitable

color

colorless

input

purified DNA

suitability

suitable for PCR

shipped in

wet ice

storage temp.

−20°C

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This Item
D5809D7442D1313
concentration

5 units/μL

concentration

2.5 unit/μL

concentration

5 unit/μL

concentration

1 unit/μL

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

color

colorless

color

colorless

color

colorless

color

red

suitability

suitable for PCR

suitability

suitable for PCR

suitability

suitable for PCR

suitability

suitable for PCR

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

AccuTaq long and accurate (LA) DNA Polymerase is a combination of Taq DNA polymerase with a small amount of a polymerase with the 3′→5′ exonuclease activity necessary for proofreading. It enables the amplification of genomic targets larger than 20 kb. This blend increases the length of amplification products by using the proofreading polymerase to repair terminal misincorporations. This repair enables the polymerase to resume elongating the growing DNA strand. After the proofreading activity, the final products mostly have a blunt end.

Application

AccuTaq LA DNA Polymerase produces long DNA amplicons for:
  • Mutation analysis
  • Cloning genes
  • DNA sequencing
  • cDNA library generation
  • Polymerase chain reaction (PCR)
  • Direct PCR

Features and Benefits

  • Increased fidelity, up to 6.5× that of Taq DNA polymerase
  • Efficiently and accurately produce amplicons up to 22 kb on genomic templates and up to 40 kb on less complex templates such as lambda or bacterial DNA.

Packaging

The enzyme is provided with an optimized 10× reaction buffer for enhanced amplification of complex templates. A separate vial of DMSO is included. Addition of DMSO in the reaction at a final concentration of 1-4% may increase yield and improve reliability of the system with some complex PCR targets.

Other Notes

AccuTaq LA DNA polymerase is an optimized blend of Sigma′s high quality Taq DNA polymerase and a small amount of an additional polymerase that exhibits 3′→5′ exonuclease or proofreading activity. By blending the Taq with the right amount of this proofreading enzyme, misincorporation errors are corrected, producing PCR amplicons that are longer and more accurate.
Store AccuTaq LA DNA Polymerase and the 10x Buffer at –20 °C. Store DMSO at room temperature. Melting frozen DMSO at ~30 °C will not affect performance.

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74°C.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
AccuTaq is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C


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Protocols

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.

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