MilliporeSigma
All Photos(1)

E2257

Sigma-Aldrich

β-Estradiol

powder, γ-irradiated, suitable for cell culture

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
1,3,5-Estratriene-3,17β-diol, 17β-Estradiol, 3,17β-Dihydroxy-1,3,5(10)-estratriene, Dihydrofolliculin
Empirical Formula (Hill Notation):
C18H24O2
CAS Number:
Molecular Weight:
272.38
Beilstein:
1914275
EC Number:
MDL number:
PubChem Substance ID:

biological source

synthetic (organic)

Quality Level

sterility

γ-irradiated

product line

BioXtra

Assay

≥98% (HPLC)

form

powder

technique(s)

cell culture | mammalian: suitable

mp

176-180 °C (lit.)

solubility

ethanol: 1 mg/mL, clear, colorless to very faintly yellow

suitability

suitable for

shipped in

ambient

storage temp.

room temp

SMILES string

O[C@H]1CC[C@@]2([H])[C@]3([H])CCC4=CC(O)=CC=C4[C@@]3([H])CC[C@@]21C

InChI

1S/C18H24O2/c1-18-9-8-14-13-5-3-12(19)10-11(13)2-4-15(14)16(18)6-7-17(18)20/h3,5,10,14-17,19-20H,2,4,6-9H2,1H3/t14-,15-,16+,17+,18+/m1/s1

InChI key

VOXZDWNPVJITMN-ZBRFXRBCSA-N

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
E2758E8875PHR1353
β-Estradiol powder, γ-irradiated, suitable for cell culture

Sigma-Aldrich

E2257

β-Estradiol

β-Estradiol BioReagent, powder, suitable for cell culture

Sigma-Aldrich

E2758

β-Estradiol

β-Estradiol ≥98%

Sigma-Aldrich

E8875

β-Estradiol

Estradiol Pharmaceutical Secondary Standard; Certified Reference Material

Supelco

PHR1353

Estradiol

sterility

γ-irradiated

sterility

-

sterility

non-sterile

sterility

-

product line

BioXtra

product line

BioReagent

product line

-

product line

-

assay

≥98% (HPLC)

assay

≥98% (HPLC)

assay

≥98%

assay

-

form

powder

form

powder

form

powder

form

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

General description

β-estradiol is a natural human estrogen. It is important for the growth of breast and reproductive epithelia, maturation of long bones and the development of secondary sexual characteristics. Estradiol is produced by immature germ cells, spermatozoa, Leydig cells and Sertoli cells. It plays a crucial role in proliferation, regulation of ion transport and regulation of apoptosis in Sertoli cells. Estradiol regulates sperm concentration, motility and morphology. β-estradiol functions as a menopausal hormone. It is implicated in replacement therapy for female hypogonadism or primary ovarian failure. Reduced levels of β-estradiol are associated with heart disease and osteoporosis.

Application

β-Estradiol is used to study cell differentiation and transformations (tumorigenicity).
β-estradiol has been used:
  • for primary culture of mouse endometrial stromal cells (ESCs)
  • for estrogen treatment in vitro
  • in the in vitro maturation of cumulus–oocyte complexes (COCs)

Biochem/physiol Actions

The major estrogen secreted by the premenopausal ovary. Estrogens direct the development of the female phenotype in embryogenesis and during puberty by regulating gene transcription and, thus, protein synthesis. It also induces the production of gonadotropins which, in turn, induce ovulation. Exposure to estradiol increases breast cancer incidence and proliferation.

Physical form

powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw

Reconstitution

To prepare 20 μg/ml stock solution: add 1 ml absolute ethanol; gently swirl to dissolve; add 49 ml sterile medium while mixing.

Pictograms

Health hazardEnvironment

Signal Word

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Lact. - Repr. 1A

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 8

1 of 8

Hydrocortisone BioReagent, suitable for cell culture

Sigma-Aldrich

H0888

Hydrocortisone

17β-Estradiol Most potent mammalian estrogenic hormone.

Sigma-Aldrich

3301

17β-Estradiol

Estradiol United States Pharmacopeia (USP) Reference Standard

USP

1250008

Estradiol

Progesterone powder, BioReagent, suitable for cell culture

Sigma-Aldrich

P8783

Progesterone

α-Estradiol powder, ≥98% (TLC)

Sigma-Aldrich

E8750

α-Estradiol

Nicotinamide BioReagent, suitable for cell culture, suitable for insect cell culture

Sigma-Aldrich

N0636

Nicotinamide

DHEA improves the antioxidant capacity of endometrial stromal cells and improves endometrium receptivity via androgen receptor
Qin A, et al.
Eur. J. Obstet. Gyn. Rep. Biol., 198, 120-126 (2016)
Improving in vitro maturation and pregnancy outcome in cattle using a novel oocyte shipping and maturation system not requiring a CO2 gas phase
Barcelo-Fimbres M, et al.
Theriogenology, 84(1), 109-117 (2015)
Estrogen preserves Fas ligand levels by inhibiting microRNA-181a in bone marrow-derived mesenchymal stem cells to maintain bone remodeling balance
Shao B, et al.
Faseb Journal, 29(9), 3935-3944 (2015)
Determination of 17 beta-estradiol in pharmaceutical preparation by UV spectrophotometry and high performance liquid chromatography methods
Yilmaz B and Kadioglu Y
Arabian Journal of Chemistry, 10, S1422-S1428 (2017)
Samantha L P Schilit et al.
American journal of human genetics, 106(1), 41-57 (2019-12-24)
Unexplained infertility affects 2%-3% of reproductive-aged couples. One approach to identifying genes involved in infertility is to study subjects with this clinical phenotype and a de novo balanced chromosomal aberration (BCA). While BCAs may reduce fertility by production of unbalanced

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service