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F2637

Sigma-Aldrich

Ficoll® 400

BioXtra, for molecular biology, lyophilized powder

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Synonym(s):
Polysucrose 400, Ficoll 400
CAS Number:
MDL number:
NACRES:
NA.52

biological source

non-animal source

Quality Level

grade

Molecular Biology
for molecular biology

product line

BioXtra

form

lyophilized powder

foreign activity

DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected

storage temp.

room temp

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This Item
F1418F8636F9378
Sigma-Aldrich

Sigma-Aldrich

F2637

Ficoll® 400

-
Ficoll® 400 BioXtra, Type 400-DL, lyophilized powder

Sigma-Aldrich

F1418

Ficoll® 400

Premium Grade
Sigma-Aldrich

Sigma-Aldrich

F8636

Ficoll® 400

-
Ficoll® 400 Type 400-DL, lyophilized powder

Sigma-Aldrich

F9378

Ficoll® 400

-
grade

Molecular Biology, for molecular biology

grade

-

grade

-

grade

-

product line

BioXtra

product line

BioXtra

product line

BioXtra

product line

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

room temp

storage temp.

15-25°C

storage temp.

-

storage temp.

15-25°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Ficoll® 400 is a non-ionic synthetic polymer of sucrose used for cell separation and organ isolation.

Application

Suitable for
  • hybridization experiments to reduce non-specific binding of labeled probes to the membrane
  • preparation of DNA loading buffer (15% Ficoll®, 0.25% bromophenol blue and 0.25% xylene cyanol FF
  • separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood)
  • producing density gradients to separate subcellular components
  • vitrification of cell and tissue samples.
  • to compare the effect of different perfusates on Hypoxic pulmonary vasoconstriction (HPV).
Used for cell separation and organelle isolation.

Features and Benefits

  • free from DNases, RNases, phosphatases and proteases
  • preserves functional and morphological integrity of the separated cells

Other Notes

A nonionic synthetic polymer of sucrose.

Legal Information

Ficoll is a registered trademark of Cytiva

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Prion-like proteins form multivalent assemblies and phase separate into membraneless organelles. Heterogeneous ribonucleoprotein D-like (hnRNPDL) is a RNA-processing prion-like protein with three alternative splicing (AS) isoforms, which lack none, one, or both of its two disordered domains. It has been
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Pulmonary circulation, 3(2), 396-405 (2013-09-10)
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Protocols

Ficoll 400® is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400® is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400® is very hydrophilic and extremely water-soluble.

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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