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F8192

Sigma-Aldrich

Fetal Bovine Serum

Australia origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for hybridoma

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Synonym(s):
HI FBS, HI sera, HI serum, FBS, FCS, sera, serum
MDL number:

biological source

bovine fetus

Quality Level

sterility

sterile-filtered

product line

FBS Classic

composition

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

origin

Australia origin

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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This Item
F8067F617812103C
Fetal Bovine Serum Australia origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for hybridoma

Sigma-Aldrich

F8192

Fetal Bovine Serum

Fetal Bovine Serum New Zealand origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

Sigma-Aldrich

F8067

Fetal Bovine Serum

Fetal Bovine Serum USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

Sigma-Aldrich

F6178

Fetal Bovine Serum

Fetal Bovine Serum USA origin, suitable for cell culture

Sigma-Aldrich

12103C

Fetal Bovine Serum

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

composition

Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL

composition

Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL

composition

Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤25 mg/dL

composition

hemoglobin, ≤20 mg/dL

origin

Australia origin

origin

New Zealand origin

origin

USA origin

origin

USA origin

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

impurities

≤10 EU/mL endotoxin

impurities

≤25 EU/mL endotoxin

impurities

≤10 EU/mL endotoxin

General description

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

Application

FBS derived from clotted blood is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.

Preparation Note

Prepared by heating at 56°C for 30 minutes.

This product is prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes inactivates the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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