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P5493

Sigma-Aldrich

Phosphate buffered saline

10× concentrate, BioPerformance Certified, suitable for cell culture

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Synonym(s):
PBS
MDL number:
eCl@ss:
32129211

grade

BioPerformance Certified
for molecular biology

Quality Level

sterility

0.2 μm filtered

form

solution

storage condition

(Tightly closed)

technique(s)

cell culture | mammalian: suitable

impurities

Bioburden, tested
endotoxin, tested
≤5 ppm heavy metals (as Pb)

color

colorless

pH

7.2-7.6 (25 °C)

solubility

water: soluble

cation traces

heavy metals (as Pb): ≤5 ppm

suitability

suitable for

foreign activity

DNAse, none detected
NICKase, none detected
Protease, none detected
RNAse, none detected

SMILES string

OP(=O)(O)[O-].OP(=O)([O-])[O-].[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]

InChI

InChI=1S/2ClH.2K.3Na.2H3O4P/c;;;;;;;2*1-5(2,3)4/h2*1H;;;;;;2*(H3,1,2,3,4)/q;;5*+1;;/p-5

InChI key

LOKCTEFSRHRXRJ-UHFFFAOYSA-I

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This Item
P5368P2272P4417
Sigma-Aldrich

Sigma-Aldrich

P5493

Phosphate buffered saline

Premium Grade
Sigma-Aldrich

Sigma-Aldrich

P5368

Phosphate buffered saline

Premium Grade
sterility

0.2 μm filtered

sterility

-

sterility

sterile-filtered

sterility

-

form

solution

form

powder

form

liquid

form

tablet

storage condition

(Tightly closed)

storage condition

(Keep container tightly closed in a dry and well-ventilated place.)

storage condition

-

storage condition

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

impurities

Bioburden, tested, endotoxin, tested, ≤5 ppm heavy metals (as Pb)

impurities

BioBurden, tested, endotoxin, tested

impurities

Endotoxin, tested

impurities

-

Application

Phosphate buffered saline has been used:
  • as a cell wash solution in human dental pulp stem cells (DPSCs)
  • for perfusion studies in dissected brain samples
  • as wash solution for neurons culture cells sample preparation for immunofluorescence and immunocytochemical labelling
  • as wash solution for ovary and sperm during in vitro culture and fertilization

Packaging

4L package size comes in a box with a spigot for spill free dispensing.

Other Notes

When diluted to a 1X concentration, this product will yield a phosphate buffered saline solution with a phosphate buffer concentration of 0.01M and a sodium chloride concentration of 0.154M. The solution pH will be 7.4.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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25G
Pack Size/Quantity

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705578-5MG-PW

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