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SHC002

Sigma-Aldrich

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA

Targets no known mammalian genes

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Synonym(s):
MISSION® Control Vectors
MDL number:
NACRES:
NA.51

product line

MISSION®

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

shipped in

dry ice

storage temp.

−20°C

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SHC005SHC007SHC004
concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

MISSION® pLKO.1-puro non-mammalian shRNA control plasmid DNA has been used:
  • to transduce RLE-6TN cells
  • for the validation of Oct4-green fluorescent protein (GFP) mouse embryonic fibroblasts (MEFs)
  • as a negative control for RNA knockdown

MISSION®pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA has been used:
  • for the knockdown of transmembrane prostate androgen-induced RNA (TMEPAI)
  • in the infection mixture for lentivirus preparation
  • for generating the puromycin-resistant ST14A (rat striatal neuronal cell line) cell populations
  • to induce Ago2 (argonaute-2) down-regulation

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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T1503
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-
25G
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705578-5MG-PW

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Identification of a gene expression signature associated with the metastasis suppressor function of NME1: prognostic value in human melanoma
Leonard MK, et al.
Laboratory Investigation; a Journal of Technical Methods and Pathology, 98(3), 327-327 (2018)
TMEPAI/PMEPA 1 enhances tumorigenic activities in lung cancer cells
Vo Nguyen TT, et al.
Cancer Science, 105(3), 334-341 (2014)
SFPQ?NONO and XLF function separately and together to promote DNA double-strand break repair via canonical nonhomologous end joining.
Jaafar l
Nucleic Acids Research, 45(4), 1848-1859 (2017)
Mutant huntingtin alters cell fate in response to microtubule depolymerization via the GEF-H1-RhoA-ERK pathway
Varma H, et al.
The Journal of Biological Chemistry, 285(48), 37445-37457 (2010)
TRIM28 is an epigenetic barrier to induced pluripotent stem cell reprogramming
Miles DC, et al.
Stem Cells, 35(1), 147-157 (2017)

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