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SHC002H

Sigma-Aldrich

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles, High Titer

Targets no known mammalian genes

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Synonym(s):
MISSION®, MISSION® Control Transduction Particles
NACRES:
NA.51

Quality Level

100
200

product line

MISSION®

concentration

≥1x109 VP/ml (via p24 assay)

technique(s)

capture ELISA: 109 TU/mL using p24

shipped in

dry ice

storage temp.

−70°C

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SHC002VSHC007VSHC003H
product line

MISSION®

product line

MISSION®

product line

MISSION®

product line

MISSION®

technique(s)

capture ELISA: 109 TU/mL using p24

technique(s)

capture ELISA: 106 TU/mL using p24

technique(s)

capture ELISA: 106 TU/mL using p24

technique(s)

capture ELISA: 109 TU/mL using p24

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

Quality Level

100, 200

Quality Level

100, 200

Quality Level

200

Quality Level

100, 200

General description

This shRNA non-mammalian control was designed using our Turbo GFP sequence and may cause some knockdown of tGFP. For maximum knockdown of tGFP, please refer to SHC004, SHC004V, SHC004H, SHC204, or SHC204V.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.

Components

High titer controls are provided as 10 x 20 μl aliquots.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Nicole T Watt et al.
Diabetes, 66(11), 2808-2821 (2017-08-24)
Shc homology 2-containing inositol 5' phosphatase-2 (SHIP2) is a lipid phosphatase that inhibits insulin signaling downstream of phosphatidylinositol 3-kinase (PI3K); its role in vascular function is poorly understood. To examine its role in endothelial cell (EC) biology, we generated mice
Mehdi Ellouze et al.
European journal of immunology, 50(4), 589-602 (2019-12-17)
Studies support the beneficial effects of glucocorticoids (GCs) during septic shock, steering research toward the potential role of GC-induced proteins in controlling excessive inflammatory responses. GILZ is a glucocorticoid-induced protein involved in the anti-inflammatory effects of GCs. We investigated whether
Min Ling et al.
Cell & bioscience, 7, 27-27 (2017-05-27)
Bone degenerative disorders like osteoporosis may be initiated by age-related shifts in anabolic and catabolic responses that control bone homeostasis. Although there are studies suggesting that metabolic changes occur with stem cell differentiation, the molecular mechanisms governing energy metabolism and
Karin M E Andersson et al.
Oncotarget, 6(24), 20043-20057 (2015-09-08)
Follicular T helper (Tfh) cells are recognized by the expression of CXCR5 and the transcriptional regulator Bcl-6. Tfh cells control B cell maturation and antibody production, and if deregulated, may lead to autoimmunity. Here, we study the role of the

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