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MCF7 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86012803, human breast (adenocarcinoma), Epithelial-like

Synonym(s):

IBMF-7 Cells, MCF7 Cells, Michigan Cancer Foundation-7 Cells

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About This Item

eCl@ss:
32190102
UNSPSC Code:
41106514
Biological source:
human breast (adenocarcinoma)
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
2n = 46, hypertriploid to hypotetraploid
Morphology:
Epithelial-like

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biological source

human breast (adenocarcinoma)

packaging

tube of 5 μg 86012803-DNA-5UG, pkg of vial of cells 86012803-1VL

growth mode

Adherent

karyotype

2n = 46, hypertriploid to hypotetraploid

morphology

Epithelial-like

products

Not specified

receptors

Reported to express the oestrogen and progesterone receptor

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Application

Tumourigenicity and virus studies: Type B and C viruses

Biochem/physiol Actions

Established from the pleural effusion from a 69 year female caucasian suffering from a breast adenocarcinoma. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of domes. Cells may carry B or C type retrovirus and are considered to represent a category 2 pathogen (P2 containment). Cells express both the wild type and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2.
Human Caucasian breast adenocarcinoma
STR-PCR Data:
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids NEAA + 10% Foetal Bovine Serum FBS / FCS.
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% Trypsin or Trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

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