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MCF7 Cell Line human

86012803, from human breast(adenocarcinoma)

Synonym(s):
IBMF-7 Cells, MCF7 Cells, Michigan Cancer Foundation-7 Cells, ssMCF-7 Cells
eCl@ss:
32190102

biological source

human breast (adenocarcinoma)

growth mode

Adherent

karyotype

2n = 46, hypertriploid to hypotetraploid

morphology

Epithelial-like

products

Not specified

receptors

Reported to express the oestrogen and progesterone receptor

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

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SCC100CB_92020424CB_93112519
MCF7 Cell Line human 86012803, from human breast(adenocarcinoma)

MCF7 Cell Line human

MDA-MB-231 Cell Line human 92020424, human breast adenocarcinoma

MDA-MB-231 Cell Line human

A2780 Cell Line human 93112519, ovarian carcinoma

A2780 Cell Line human

growth mode

Adherent

growth mode

-

growth mode

Adherent

growth mode

Adherent

karyotype

2n = 46, hypertriploid to hypotetraploid

karyotype

-

karyotype

Modal no.′s 62 and 64, near triploid

karyotype

Not specified

morphology

Epithelial-like

morphology

-

morphology

Epithelial

morphology

Epithelial

products

Not specified

products

-

products

Not specified

products

Not specified

receptors

Reported to express the oestrogen and progesterone receptor

receptors

-

receptors

Not specified

receptors

Not specified

Cell Line Origin

Human Caucasian breast adenocarcinoma

Cell Line Description

Established from the pleural effusion from a 69 year female caucasian suffering from a breast adenocarcinoma. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of domes. Cells may carry B or C type retrovirus and are considered to represent a category 2 pathogen (P2 containment). Cells express both the wild type and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2.

Application

Tumourigenicity and virus studies: Type B and C viruses

DNA Profile

STR-PCR Data:
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids NEAA + 10% Foetal Bovine Serum FBS / FCS.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% Trypsin or Trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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