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MDA-MB-231 Cell Line human

92020424, human breast adenocarcinoma

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Synonym(s):
MD Anderson-Metastatic Breast-231 Cells, MDA-MB231 Cells, MDA231-BRE Cells, MDAMB231 Cells
eCl@ss:
32190102

biological source

human breast

growth mode

Adherent

karyotype

Modal no.′s 62 and 64, near triploid

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human Caucasian breast adenocarcinoma

Cell Line Description

Isolated from pleural effusions of a breast cancer patient.

Application

MDA-MB-231 has been used to study:
  • the changes in the morphological pattern and gene expression of cells when grown in the presence of sera positive for anti-carbonic anhydrase I (CA I) autoantibodies
  • the effects of normothermic conditioned microwave irradiation on cultured cells using an irradiation system
  • the transendothelial migration rates of the cells under different shear stress levels

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 12,13
D13S317: 13
D16S539: 12
D5S818: 12
D7S820: 8,9
THO1: 7,9.3
TPOX: 8,9
vWA: 15,18

Culture Medium

L15+ 2mM Glutamine + 15% Foetal Bovine SerumFBS / FCS.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e. seeding at 3-6x10,000 cells/cm2 using 0.05% Trypsin or Trypsin/EDTA; 37°C. CO2 is not required.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

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Articles

DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.

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