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LNCap Clone FGC Cell Line human

from human prostate(carcinoma), 89110211

Synonym(s):

LNCaP-FGC Cells

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About This Item

UNSPSC Code:
41106514

biological source

human prostate (carcinoma)

growth mode

Adherent

karyotype

Pseudodiploid male; seven marker chromosomes, modal number 46, range 33 to 91

morphology

Epithelial-like

products

Prostate acid phosphatase, prostate specific antigen

receptors

Androgen, estrogen

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

metastasis

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human Caucasian prostate carcinoma

Cell Line Description

Derived from a metastasis at the left supraclavicular lymph node of a 50 year old patient with a confirmed diagnosis of metastatic prostate carcinoma. Growth and acid phosphatase production is affected by 5-alpha-dihydrotestosterone. They do not form a uniform monolayer and attach only lightly to the substrate. When shipped, cells detach from flask and can either be incubated 24-48 hours to allow attachment or be collected by centrifugation (150xg, 15 minutes) and reseeded.

Application

LNCap Clone FGC Cell Line has been used:
  • to study the effect of sex steroid hormones in regulating the expression of oligoadenylate synthetase 1 (OAS1)
  • to study the role of curcumin and resveratrol in redox control and ability to promote apoptosis
  • to investigate low molecular weight thiols present in the deproteinized extract of the cell line after derivatization

Culture Medium

RPMI 1640 + 2mM Glutamine + 1.0 mM sodium pyruvate + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.i.e. seeding at 1-3 x 10,000 cells/cm2 Cells grow slowly in clusters and can be disaggregated by repeated pipetting. After subculture cells may take up to 48 hours to reattach. Do not disturb during this period. Medium is rapidly acidified, therefore change twice per week.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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