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RAW 264.7 Cell Line murine

91062702, Macrophage from blood

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Synonym(s):
RAW264 7 Cells, RAW264.7 Cells

biological source

mouse blood

growth mode

Semi-adherent

karyotype

Not specified

morphology

Macrophage

products

Lysozyme

receptors

Immunoglobulin, complement

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Mouse monocyte macrophage

Cell Line Description

Established from an ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells will pinocytose neutral red and phagocytose zymosan. Cells capable of antibody dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS.

Application

Metabolic studies
RAW 264.7 cell line has been used to study the anti-inflammatory effects of pristimerin. It has also been used to test the internalization and intracellular survival of Listeria monocytogenes.

Culture Medium

DMEM + 2mM Glutamine + 10% FBS / FCS (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-4x10,000 cells/cm2; 5% CO2; 37°C. Remove the cells mechanically. Cells are semi-adherent, i.e. some cells grow in suspension, some loosely attach to the surface and others flattened out and attached to the flask. Cells should not be allowed to overgrow and become confluent as this can lead to loss of the flattened adherent cell characteristic.

Other Notes

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