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P1118

Sigma-Aldrich

pT7-FLAG™-1 Expression Vector

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About This Item

UNSPSC Code:
12352200

grade

Molecular Biology
for molecular biology

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

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This Item
C4478B8931B2904
S-Gal® reagent for selection of recombinant bacterial clones

S9811

S-Gal®

S-Gal®/LB Agar Blend reagent for selection of recombinant bacterial clones

C4478

S-Gal®/LB Agar Blend

Magenta-Gal reagent for selection of recombinant bacterial clones

B8931

Magenta-Gal

Bluo-Gal reagent for selection of recombinant bacterial clones

B2904

Bluo-Gal

suitability

suitable for β-galactosidase test

suitability

suitable for β-galactosidase test, nonselective for Escherichia coli, nonselective for coliforms

suitability

suitable for substrate for β-galactosidase

suitability

suitable for substrate for β-galactosidase

solubility

DMF: 50 mg/mL

solubility

-

solubility

methanol: soluble

solubility

DMF: soluble

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

powder

form

powder

form

powder

General description

pT7-FLAG™-2 is a 4.8 kb E . coli expression vector used for cloning and cytoplasmic expression of a properly inserted open reading frame as a N-terminal Met-FLAG fusion protein containing the FLAG® epitope (DYKDDDDK). The promoter region of the very strong phage T7 promoter drives transcription of ORFFLAG fusion constructs. This vector requires the use of E. coli cells containing a source of the T7 polymerase, such as BL21(DE3)T1R cells, Catalog Number B2935.

Application

pT7-FLAG®-1 Expression Vector has been used in the cloning of ubiquitin fusion protein[1] and connexin 43 (Cx43) C-terminal fragment.[2]

Components

pT7-FLAG™-1 Expression Vector (P6742) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
pT7-FLAG™-1-BAP Control Vector (P6992) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pT7-FLAG is a trademark of Sigma-Aldrich Co. LLC

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Storage Class Code

12 - Non Combustible Liquids

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    SLBP3485V
    SLBN4766V

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    Chase T Archer et al.
    The Journal of biological chemistry, 283(31), 21789-21798 (2008-06-03)
    Destabilization of activator-DNA complexes by the proteasomal ATPases can inhibit transcription by limiting activator interaction with DNA. Modification of the activator by monoubiquitylation protects the activator from this destabilization activity. In this study, we probe the mechanism of this protective
    Yo Sugawara et al.
    The Journal of cell biology, 189(4), 691-700 (2010-05-12)
    Botulinum neurotoxin is produced by Clostridium botulinum and forms large protein complexes through associations with nontoxic components. We recently found that hemagglutinin (HA), one of the nontoxic components, disrupts the intercellular epithelial barrier; however, the mechanism underlying this phenomenon is
    Alasdair I Macdonald et al.
    The Biochemical journal, 446(1), 9-21 (2012-06-05)
    Gap junctions, composed of Cxs (connexins), allow direct intercellular communication. Gap junctions are often lost during the development of malignancy, although the processes behind this are not fully understood. Cx43 is a widely expressed Cx with a long cytoplasmic C-terminal
    Ning Kong et al.
    Autophagy, 16(10), 1737-1752 (2019-12-24)
    Interferon-induced BST2 (bone marrow stromal cell antigen 2) inhibits viral replication by tethering enveloped virions to the cell surface to restrict viral release and by inducing the NFKB-dependent antiviral immune response. However, the mechanism by which BST2 uses the selective

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