S9811

S-Gal^®

reagent for selection of recombinant bacterial clones

• Synonym(s): 3,4-Cyclohexenoesculetin β-D-galactopyranoside
• Empirical Formula (Hill Notation): C₁₉H₂₂O₉
• CAS Number: 182805-65-8
• Molecular Weight: 394.37
• UNSPSC Code: 12352200
• PubChem Substance ID: 329824635
• NACRES: NA.85

Properties

• grade: for molecular biology
• Quality Level: 100
• sterility: non-sterile
• assay: ≥95% (HPLC)
• form: powder
• solubility: DMF: 50 mg/mL
• suitability: suitable for β-galactosidase test
• storage temp.: room temp
• SMILES string: OC[C@H]1O[C@@H](Oc2cc3OC(=O)C4=C(CCCC4)c3cc2O)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C19H22O9/c20-7-14-15(22)16(23)17(24)19(28-14)27-13-6-12-10(5-11(13)21)8-3-1-2-4-9(8)18(25)26-12/h5-6,14-17,19-24H,1-4,7H2/t14-,15+,16+,17-,19-/m1/s1
• InChI key: HDJJDRXZHJRVGA-DIKXUDHVSA-N

Description

General description

S-Gal^® is an autoclavable, chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. S-Gal^® is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Application

Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. S-Gal^® is autoclavable and can be added to bacterial broth containing agar prior to autoclaving.

S-Gal is a patented autoclavable chromogenic substrate for β-galactosidase that is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac⁺ phenotype.

Features and Benefits

• More intense color contrast than X-gal
• Excellent for use in automated colony counters
• Autoclavable for easiest use
• No need to make stock solutions

When S-Gal is cleaved by β-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac⁺ colonies grown in the presence of S-Gal and ferric ion turn an intense black color, allowing for easy differentiation between lac⁺ and lac^- colonies. S-Gal is autoclavable and can be added to your medium of choice prior to autoclaving. S-Gal is not light sensitive and does not require any protection from light sources.

Other Notes

Autoclavable, microwavable growth medium complete with S-Gal and IPTG.

The ferric or Fe³⁺ ion is required for color development and must be added to any S-Gal^®
formulation. A medium prepared with S-Gal^® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.

Principle

When S-Gal^® is cleaved by ß-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal^® and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies.

Reconstitution

Stock solutions of S-Gal^® can be made by dissolving 50mg/ml in dimethyl formamide (DMF) and storing at -20C. Add S-Gal^® (300 mg/L from stock solution ) and Ferric Ammonium Citrate (500mg/L) to agar media prior to autoclaving.

Legal Information

S-GAL is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves