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XGAL-RO

Roche

X-Gal

solubility: in DMF, soluble, solubility: in methanol, soluble, storage temp.:2-8°C

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Synonym(s):
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside, bromo-4-chloro-3-indolyl-β-d-galactopyranoside, 5-, 5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal
Empirical Formula (Hill Notation):
C14H15BrClNO6
CAS Number:
Molecular Weight:
408.63
Beilstein:
1402009
MDL number:
PubChem Substance ID:

description

5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside

Quality Level

packaging

pkg of 1 g (10745740001)
pkg of 100 mg (03117073001)
pkg of 100 mg (11680293001)
pkg of 2.5 g (10703729001)
pkg of 250 mg (10651745001)

manufacturer/tradename

Roche

solubility

DMF: soluble
methanol: soluble

storage temp.

2-8°C

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

InChI key

OPIFSICVWOWJMJ-AEOCFKNESA-N

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This Item
B9146203782B6024
X-Gal solubility: in DMF, soluble, solubility: in methanol, soluble, storage temp.:2-8°C

Roche

XGAL-RO

X-Gal

solubility

DMF: soluble, methanol: soluble

solubility

DMF: methanol (1:1): soluble 50 mg/mL

solubility

DMSO: 2% (20mg/mL)

solubility

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

200

description

5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside

description

-

description

-

description

-

packaging

pkg of 1 g (10745740001)

packaging

-

packaging

-

packaging

-

General description

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. It is soluble in dimethylformamide and methanol. The product is not soluble in water.

Application

X-Gal has been used for immunohistocytochemistry applications. It is also the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.
X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.

Quality

Chromatographically homogeneous

Physical form

Crystals, white powder

Preparation Note

Working solution: Stock solution:
  • 20 mg/ml in dimethyl formamide (DMF), clear, colorless solution
  • A590 nm: 0.01

Storage conditions (working solution): X-gal in solution (i.e., dissolved in DMF 2% (w/v)) is stable for several weeks when stored in the dark at 2 to 8 °C; when the solution is stored at -15 to -25 °C, it is stable for one year.

Other Notes

For life science research only. Not for use in diagnostic procedures.

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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