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GALS

Sigma-Aldrich

β-Galactosidase Reporter Gene Staining Kit

Synonym(s):

β-galactosidase, LacZ

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.32
Pricing and availability is not currently available.

usage

 kit sufficient for 100 tests (using a 3.5 cm dish)

Quality Level

200

technique(s)

microbe id | staining: suitable

shipped in

dry ice

storage temp.

−20°C

General description

β-galactosidase (β-gal) catalyzes the conversion of X-gal to galactose and 5-bromo-4-chloro-3-hydroxyindole.[1] The functionality of β-gal is indirectly measured by the blue precipitate of dimeric 5-bromo-4-chloro-3-hydroxyindole in the presence of potassium ferricyanide or ferrocyanide.[1] The colorimetric detection methods also use other substrates of β-gal like O-nitrophenyl-β-D-galactopyranoside (ONPG). β-gal is a cost-effective, simple and standard reporter system.[2] The Galactosidase Reproter Gene Staining Kit provides a method for determining the percentage of cells transfected with the plasmid expressing the β-galactosidase gene, LacZ. It can also be used to visualize the specific expression of the reporter gene in tissue sections by microscopy.

Application

β-Galactosidase Reporter Gene Staining Kit has been used for histochemical staining of animal cells or tissues such as:
  • sagittal brain sections in histoenzymology[3]
  • pancreas or colon sections in β-galactosidase reporter gene assay[4]
  • mice E10.5 -11.5 embryo and embryo sections[5]

Other Notes

The substrate used in this kit is X-Gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) which results in an indigo blue-colored staining of transfected cells and tissues. This test provides a method for determining the percentage of transfected cells that are expressing LacZ or for microscopically visualizing reporter gene expression in tissue sections.

related product

Product No.
Description
Pricing

94433

β-Galactosidase stain

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

supp_hazards

EUH032

Storage Class

3 - Flammable liquids

flash_point_f

132.8 °F

flash_point_c

56 °C

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Certificates of Analysis (COA)

Lot/Batch Number
0000327711
0000177219
029M4161V
11180807
117M4124V

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