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9630-OP

Sigma-Aldrich

X-Gal

OmniPur® Grade

Synonym(s):

OmniPur® X-Gal, 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, BCIG, X-Gal

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About This Item

Empirical Formula (Hill Notation):
C14H15BrClNO6
CAS Number:
7240-90-6
Molecular Weight:
408.63
MDL number:
MFCD00005666
UNSPSC Code:
12352202
NACRES:
NA.25

Quality Level

100

form

powder

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

shipped in

ambient

InChI

1S/C19H29N5O.2C2H6O2.2Cu/c1-21-3-7-23(8-4-21)14-17-11-16(13-20)12-18(19(17)25)15-24-9-5-22(2)6-10-24;2*1-2(3)4;;/h11-12,25H,3-10,14-15H2,1-2H3;2*2-4H,1H3;;/q;;;+1;+2

InChI key

GGILAJWFDJWIQO-UHFFFAOYSA-N

General description

X-Gal is a chromogenic substrate used in conjunction with IPTG for blue/white colony selection. It is a soluble colorless compound comprising galactose attached to a substituted indole. In the presence of X-Gal the lac+ bacteria after α-complementation form blue colonies and bacteria carrying recombinant plasmid form white colonies.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
OMNIPUR is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Michael R Green et al.
Cold Spring Harbor protocols, 2019(12) (2019-12-04)
Many plasmid vectors (e.g., the pUC series, Bluescript, pGem, and their derivatives) carry a short segment of Escherichia coli DNA containing the regulatory sequences and the coding information for the first 146 amino acids of β-galactosidase. Vectors of this type
Douglas H Juers et al.
Protein science : a publication of the Protein Society, 21(12), 1792-1807 (2012-09-27)
This review provides an overview of the structure, function, and catalytic mechanism of lacZ β-galactosidase. The protein played a central role in Jacob and Monod's development of the operon model for the regulation of gene expression. Determination of the crystal

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