203782

5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside

A chromogenic substrate for β-galactosidase. Used to distinguish between recombinant and non-recombinant plasmids carrying β-galactosidase gene (e.g. pUR222).

• Synonym(s): 5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside, X-Gal
• Empirical Formula (Hill Notation): C₁₄H₁₅BrClNO₆
• CAS Number: 7240-90-6
• Molecular Weight: 408.63
• MDL number: MFCD00005666
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• assay: ≥99% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated; protect from light
• color: white
• solubility: DMSO: 2% (20mg/mL)
• shipped in: ambient
• storage temp.: −20°C
• InChI: 1S/C19H29N5O.2C2H6O2.2Cu/c1-21-3-7-23(8-4-21)14-17-11-16(13-20)12-18(19(17)25)15-24-9-5-22(2)6-10-24;2*1-2(3)4;;/h11-12,25H,3-10,14-15H2,1-2H3;2*2-4H,1H3;;/q;;;+1;+2
• InChI key: GGILAJWFDJWIQO-UHFFFAOYSA-N

Description

General description

A chromogenic substrate for β-galactosidase. Used to distinguish between recombinant and non-recombinant plasmids carrying β-galactosidase gene (e.g. pUR222).

Warning

Toxicity: Standard Handling (A)

Other Notes

Engler, K.H., et al. 1999. J. Microbiol. Methods35, 207.
Arakawa, H., et al. 1991. Anal. Biochem.199, 238.
Horwitz, J., et al. 1964. J. Med. Chem.7, 574.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable