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  • A defective HSV-1 vector expresses Escherichia coli beta-galactosidase in cultured peripheral neurons.

A defective HSV-1 vector expresses Escherichia coli beta-galactosidase in cultured peripheral neurons.

Science (New York, N.Y.) (1988-09-23)
A I Geller, X O Breakefield
ABSTRACT

A defective herpes simplex virus 1 (HSV-1) vector, pHSVlac, has been developed that contains a transcription unit that places the Escherichia coli lacZ gene under the control of the HSV-1 immediate early 4/5 promoter. The vector pHSVlac was propagated with the HSV-1 temperature-sensitive mutant ts K as helper virus. Infection of neurons from rat superior cervical ganglia and dorsal root ganglia in primary culture resulted in stable expression of high levels of beta-galactosidase without cell death. These HSV-1 vectors should be useful for introducing genes into postmitotic cells, such as neurons, in vitro and in vivo.

MATERIALS
Product Number
Brand
Product Description

Roche
X-Gal, solubility: in DMF, soluble, solubility: in methanol, soluble, storage temp.:2-8°C